Strain Data Sheet

RBRC09295

Information1

Image
BRC No.RBRC09295
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Unc5d<tm1.1>
Former Common nameUnc5D KO
H-2 Haplotype
ES Cell lineF1(C57BL/6J x 129/SvJ)
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Seiki Haraguchi, National Agriculture and Food Research Organization.
Strain descriptionExon 1 was replaced with a loxP-neo-loxP cassette. The floxed neo cassette was removed by cre excision.
Colony maintenance
ReferencesJournal of Clinical Investigation, 123(7), 2935-2947 (2013). 23778138

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Unc5dunc-5 homolog D (C. elegans)8Unc5d<tm1.1Shar>targeted mutation 1.1, Seiki Haraguchi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Information2

Donor DNAPhage P1 loxP site, mouse Unc5d genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorYuichi Wakabayashi (Chiba Cancer Center)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data