RBRC09260

Information1

Image
BRC No.RBRC09260
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Cnga2<tm1Kob>
Former Common nameCNGA2flox
H-2 Haplotype
ES Cell lineEGR-05 [129S2]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, (2015). 25564662

Health Report

Examination Date / Room / Rack2020/06/01Room:3-BRack:G
2020/02/17Room:3-BRack:G
2019/12/16Room:3-BRack:G
2019/10/21Room:3-BRack:G
2019/08/26Room:3-BRack:G
2019/06/17Room:3-BRack:G
2018/04/16Room:3-CRack:L
2018/02/19Room:3-CRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cnga2cyclic nucleotide gated channel alpha 2XCnga2<tm1Kob>targeted mutation 1, Reiko Kobayakawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)Xmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
polyAX

Information2

Donor DNAPhaga P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, polyA, mouse Cyclic nucleotide-gated channel alpha 2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 2015Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT shall contact the Developer scientist (Dr. Reiko Kobayakawa) and obtain a permission of its use.
DepositorReiko Kobayakawa (Osaka Bioscience Institute)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Mar 2017

BRC mice in Publications

No Data