RBRC09257

Information1

Image
BRC No.RBRC09257
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC.Cg-Tg(CAG-tdKaede)15Utr
Former Common nameKaede (WK-m15), BALB-CAG-Kaede Tg#15
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Michio Tomura, Kyoto University Graduate School of Medicine and Yoshihiro Miwa, Graduate School of Comprehensive Human Sciences, University of Tsukuba. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The Kaede cDNA was provided by Atsushi Miyawaki, RIKEN Brain Science Institute. The Tg mice were backcrossed to BALB/c over 22 times.
Strain descriptionThese transgenic mice expressing a Kaede, photoconvertible fluorescence protein under the control of the CAG promoter. The expression of green fluoresce of Kaede mice can be converted to red on irradiation with UV or violet light.
Colony maintenanceCarrier x Noncarrier [BALB/cAJcl]
ReferencesProc. Natl. Acad. Sci. USA, 105(31):10871-10876 (2008). 18663225

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
tdKaedeTrachyphyllia geoffroy Kaede cDNAUNtdKaedeCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Trachyphyllia geoffroy Kaede cDNA, SV40 Poly(A) site
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 105, 10871-10876 (2008).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Kaede using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: sakurai-h@brain.riken.jp). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR Dr. Yoshihiro Miwa (ymiwa@md.tsukuba.ac.jp) of University of Tsukuba and obtain a use permission.
DepositorYoshihiro Miwa (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering: 1. Approval form (form D) from the depositor (ENG) , (JPN) , 2. Approval form (form V) from the developer, 3. MTA for distribution with RIKEN BRC and 4. Order form (ENG) , (JPN) .

BRC mice in Publications

No Data