RBRC09254

Information1

Image
BRC No.RBRC09254
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm1(CAG-kikGR)Kgwa>
Former Common nameB6-ROSA/kikGR(floxed), B6-ROSA/kikGRfloxed KI
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Michio Tomura, Kyoto University Graduate School of Medicine. M1 ES cells were used to generate the mutant mice.
Strain descriptionCAG-lox-STOP-lox-kikGR ROSA26 knock-in mice. C57BL/6 background (RBRC09254), BALB/c background (RBRC09255).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesSci. Rep. e6030 (2014). 25112380

Health Report

Examination Date / Room / Rack2020/03/09Room:2-検Rack:C
2020/01/06Room:2-検Rack:C
2019/11/11Room:2-検Rack:C
2019/09/09Room:2-検Rack:C
2019/07/08Room:2-検Rack:C
2019/05/07Room:2-検Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-kikGR)Kgwa>targeted mutation 1, Osami Kanagawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
STOP6STOP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
kikGRFavia favus Kikume-Green Red cDNA6kikGRCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, Stop, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, Favia favus Kikume-Green Red cDNA, chicken poly(A) sequence, mouse Gt(ROSA)26Sor genomic region
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the kikGR using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: sakurai-h@brain.riken.jp). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a MTA regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki. Osaka University (FAX:+81-6-6879-3829, e-mail: jimiyaza@nutri.med.osaka-u.ac.jp). In publishing the research results to be obtained by the use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Tomura M et.al, Sci. Rep. e6030 (2014). The RECIPIENT must send the DEPOSITOR a reprint of the RECIPIENT's publication. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Feb 2017
Necessary documents for ordering: 1. Approval form (form D) from the depositor (ENG) , (JPN) , 2. Approval form (form V) from the developer, 3. MTA for pCAGGS with the developer (Word) , 4. MTA for distribution with RIKEN BRC and 5. Order form (ENG) , (JPN) .

BRC mice in Publications

No Data