RBRC09246

Information1

Image
BRC No.RBRC09246
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Sik3<tm1Haya>
Former Common nameSik3 floxed, B6-Sik3 floxed
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Naoto Hayasaka, Graduate School of Medicine, Yamaguchi University in 2013. RENKA ES cells were used to generate the mutant mice.
Strain descriptionSik3 gene floxed mice. Exon 3 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sik3SIK family kinase 39Sik3<tm1Haya>targeted mutation 1, Naoto Hayasaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse phosphoglycerate kinase polyA, mouse Sik3 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Metabolism Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorNaoto Hayasaka (Yamaguchi University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data