RBRC09241

Information1

Image
BRC No.RBRC09241
TypeTransgene Congenic and Spontaneous MutationCartagena
SpeciesMus musculus
Strain nameHR1.Cg-Hr<hr> Tg(EF1A-PDHL/roGFP1)121Soht
Former Common nameMito-roGFP(MROH121), HR1-hr/Mito-roGFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Alexander M. Wolf, Nippon Medical School in 2012. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mutant mice were backcrossed to Hos:HR-1 over 10 generations.
Strain descriptionTransgenic mice expressing ratiometric redox-sensitive GFP (roGFP) in the mitochondria of several tissues, including skin epidermal keratinocytes. Hairless mice background.
Colony maintenanceTg: Carrier x Noncarrier; hr: Homozygote x Homozygote [or Crossing to Hos:HR-1]
ReferencesJ. Invest. Dermatol., 134(6), 1701-1709 (2014). 24129062

Health Report

Examination Date / Room / Rack2019/05/27Room:4-CRack:E
2019/03/25Room:4-CRack:E
2019/01/28Room:4-CRack:E
2018/11/26Room:4-CRack:E
2018/09/25Room:4-CRack:E
2018/07/30Room:4-CRack:E
2018/05/21Room:4-CRack:E
2018/03/26Room:4-CRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hrhairless14Hr<hr>hairless

Gene symbolGene nameChr.Allele symbolAllele namePromoter
CSF3human G-CSF cDNA PolyA signalUNCSF3

Gene symbolGene nameChr.Allele symbolAllele namePromoter
PDHA1mitchondrial targeting seaquence (human pyruvate dehydrogenase E1 subunit leader sequence)UNPDHA1human elongation factor 1 alpha (EF-1 alpha) promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
roGFPRedox-sensitive Green Fluorescent Protein (Jellyfish)UNroGFP

Information2

Donor DNAhuman EF1A promoter, mitchondrial targeting seaquence (human pyruvate dehydrogenase E1 subunit leader sequence, PDHA1), jellyfish GFP(EGFP) cDNA, human G-CSF cDNA PolyA signal
Research applicationDermatology Research
Fluorescent Proteins/lacZ System
Metabolism Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Invest. Dermatol.,134(6), 1701-1709 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigeo Ohta (Nippon Medical School)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data