Strain Data Sheet

RBRC09240

Information1

Image
BRC No.RBRC09240
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Zeb2<tm2.1Yhi>
Former Common nameSIP1-EGFP, Zfhx1b/GFP KI, STOCK Zfhx1b<tm2.1Yhi>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yujiro Higashi, Aichi Human Service Center in 2008. R1 ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with CAG-Cre mice (B6). ICR mixed background (RBRC09239), ICR and C57BL/6 mixed background (RBRC09240).
Strain descriptionSIP1 (Zeb2, Zfhx1b) gene GFP knock-in mice. A EGFP was inserted in-frame with the coding sequence in exon 9 of the Zeb2 gene.
Colony maintenanceHeterozygote x Wild-type
ReferencesGenesis, 52, 56-67 (2014). 24243579

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Zeb2zinc finger E-box binding homeobox 22Zeb2<tm2.1Yhi>targeted mutation 2.1, Yujiro Higashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)2EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Information2

Donor DNABacteriophage P1 loxP site, Jellyfish EGFP cDNA, mouse Zeb2 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
Fluorescent Proteins/lacZ System
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 52, 56-67 (2014).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYujiro Higashi (Aichi Prefectural Colony)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data