Strain Data Sheet

RBRC09239

Information1

Image
BRC No.RBRC09239
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;129(Cg)-Zeb2<tm2.1Yhi>
Former Common nameICR-SIP1-EGFP, ICR-Zfhx1b/GFP KI
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yujiro Higashi, Aichi Human Service Center in 2008. R1 ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with CAG-Cre mice (B6). ICR mixed background (RBRC09239), ICR and C57BL/6 mixed background (RBRC09240).
Strain descriptionSIP1 (Zeb2, Zfhx1b) gene GFP knock-in mice. A EGFP was inserted in-frame with the coding sequence in exon 9 of the Zeb2 gene.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Slc:ICR]
ReferencesGenesis, 52, 56-67 (2014). 24243579

Health Report

Examination Date / Room / Rack2015/12/14Room:3-BRack:L
2015/10/19Room:3-BRack:L
2015/08/17Room:3-BRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Zeb2zinc finger E-box binding homeobox 22Zeb2<tm2.1Yhi>targeted mutation 2.1, Yujiro Higashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)2EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Information2

Donor DNABacteriophage P1 loxP site, Jellyfish EGFP cDNA, mouse Zfhx1b genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
Fluorescent Proteins/lacZ System
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 52, 56-67 (2014).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYujiro Higashi (Aichi Prefectural Colony)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Jul 2015
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data