Strain Data Sheet

RBRC09239

Strain Information

Image
BRC No.RBRC09239
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;129(Cg)-Zeb2<tm2.1Yhi>
Former Common nameICR-SIP1-EGFP, ICR-Zfhx1b/GFP KI
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yujiro Higashi, Aichi Human Service Center in 2008. R1 ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with CAG-Cre mice (B6). ICR mixed background (RBRC09239), ICR and C57BL/6 mixed background (RBRC09240).
Strain descriptionSIP1 (Zeb2, Zfhx1b) gene GFP knock-in mice. A EGFP was inserted in-frame with the coding sequence in exon 9 of the Zeb2 gene.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Slc:ICR]
References
SIP1 expression patterns in brain investigated by generating a SIP1-EGFP reporter knock-in mouse.
Nishizaki Y, Takagi T, Matsui F, Higashi Y
Genesis, 52, 56-67 (2014). 24243579

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)2EGFP
Zeb2
MGI:1344407		zinc finger E-box binding homeobox 22Zeb2<tm2.1Yhi>
MGI:5574184		targeted mutation 2.1, Yujiro Higashi
  • Mowat-Wilson syndrome(DisGeNET, MedGEN)
    • loxP phage P1 loxP2loxP

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • obsolete no phenotypic analysis (MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP site, Jellyfish EGFP cDNA, mouse Zfhx1b genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 52, 56-67 (2014). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorYujiro Higashi (Aichi Prefectural Colony)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jul 2015

    BRC mice in Publications

    No Data