Strain Data Sheet

RBRC09237

Strain Information

Image
BRC No.RBRC09237
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.ICR-Tg(Cdkn1a-luc)34Eiha/EihaRbrc
Former Common namep21-p-luc(B6), B6-p21-p-luc Tg, p21-p-luc(Acc. No. CDB0415T-34)(B6)
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Eiji Hara, The Cancer Institute, Japanese Foundation for Cancer Research. The transgene was injected into the pronuclei of ICR fertilized eggs. The mutant mice were backcrossed to C57BL/6.
Strain descriptionhuman p21-luciferase transgenic mice.
Colony maintenanceCarrier x Noncarrier [C57BL/6Jcl]
References
Visualizing the dynamics of p21(Waf1/Cip1) cyclin-dependent kinase inhibitor expression in living animals.
Ohtani N, Imamura Y, Yamakoshi K, Hirota F, Nakayama R, Kubo Y, Ishimaru N, Takahashi A, Hirao A, Shimizu T, Mann D J, Saya H, Hayashi Y, Arase S, Matsumoto M, Kazuki N, Hara E
Proc. Natl. Acad .Sci. USA, 104, 15034-15039 (2007). 17848507

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 late polyA signalUN
lucluciferaseUNluchuman CDKN2A promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman CDKN2A(p21<Waf1/Cip1>) promoter, Firefly luciferase cDNA, SV40 late polyA signal
    Research applicationCancer Research
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Eiji HARA (hara@genome.tokushima-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0415T-34 and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, Proc Natl Acad Sci U S A. 2007 Sep 18; 104 (38): 15034-9. (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data