RBRC06806

Information1

Image
BRC No.RBRC06806
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(hOX-EGFP,-cre)1Ahky
Former Common nameOrexin-EGFP-2A-Cre, B6-hOX-GFP/Cre Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Akihiro Yamanaka, Research Institute of Environmental Medicine Nagoya University in 2014. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain descriptionOrexin-EGFP-2A-Cre transgenic mice. Tg mice show no obvious abnormality.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesNeuropharmacology, 85, 451-460 (2014). 24951857

Health Report

Examination Date / Room / Rack2020/03/30Room:4-ARack:C
2020/01/27Room:4-ARack:C
2019/11/25Room:4-ARack:C
2019/09/30Room:4-ARack:C
2019/07/29Room:4-ARack:C
2019/05/27Room:4-ARack:C
2019/03/25Room:4-ARack:C
2019/01/28Room:4-ARack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)XEGFPhuman prepro-orexin promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
F2AFoot-and-Mouth Disease Virus (FMDV) 2A peptideXF2A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinaseXcre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
protamine intron, polyA (mouse)X

Information2

Donor DNAhuman prepro-orexin promoter, jellyfish EGFP cDNA, Foot-and-mouth disease virus 2A peptide, Phage P1 Cre, mouse protamine intron and polyA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For profit use of the BIOLOGICAL RESSOURCE, the RECIPIENT must contact with the Intellectual Property Office, Nagoya University to obtain a prior written consent.RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkihiro Yamanaka (Nagoya University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data