RBRC06805

Information1

Image
BRC No.RBRC06805
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1(dach1)Hhsk>
Former Common nameDach Tg, B6-ROSA26/Dach1 KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Haruko Hayasaka, Graduate School of Medicine, Osaka University. RENKA ES cells were used to generate the mutant mice.
Strain descriptionDach1 ROSA26 conditional knock-in mice. Targeted insertion of SA-loxP-CAT-PGK-neo-loxP-Dach1-pA cassette into the mouse Rosa26 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(dach1)Hhsk>targeted mutation 1, Haruko Hayasaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dach1dachshund 1 (Drosophila)6Dach1<tm1Hhsk>targeted mutation 1, Haruko Hayasaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptor (adenovirus)6SA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
catE. coli chloramphenicol acetyltransferase (CAT)6

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal6

Information2

Donor DNAPhage P1 loxP sites, E. coli cat, bovine growth hormone polyA signal, mouse Dachshund1 cDNA, adenovirus type2 Splicing acceptor (SA), yeast FRT (flipase recombination target) site, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 polyA signal, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCancer Research
Cre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT, which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not -for-profit research, is required to enter into a Material Transfer Agreement with Osaka University.
DepositorHaruko Hayasaka (Kindai University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data