RBRC06804

Information1

Image
BRC No.RBRC06804
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Dach1<tm1.1Hhsk>
Former Common nameB6-Dach1 null
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Haruko Hayasaka, Graduate School of Medicine, Osaka University. KY1.1 ES cells were used to generate the mutant mice. C57BL/6 and 129 mixed background.
Strain descriptionDach1 gene null mice. Exon 1 including 1st ATG was deleted by Cre recombinase.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dach1dachshund 1 (Drosophila)14Dach1<tm1.1Hhsk>targeted mutation 1.1, Haruko Hayasaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP14loxP

Information2

Donor DNAPhage P1 loxP site, yeast FRT (flipase recombination target) site, mouse Dach1 genomic DNA, [mouse Tie2 promoter, Phage P1 cre, mouse Mt1 polyA signal sequence, mouse Tie2 intron 1 enhancer]
Research applicationCancer Research
Cre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER, Dr. Haruko Hayasaka (hhayasaka@life.kindai.ac.jp) using the Approval Form. RECIPIENT, which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research, is required to enter into a Material Transfer Agreement with Kindai University.
DepositorHaruko Hayasaka (Kindai University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data