RBRC06588

Information1

Image
BRC No.RBRC06588
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Gt(ROSA)26Sor-C1V1/EYFP)1Imayo
Former Common nameR26 BAC::pCAG-LF-C1V1-EYFP, B6-ROSA26/C1V1-YFP/LF Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi, Institute for Virus Research, Kyoto University in 2012. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptionC1V1-EYFP(E122T/E162T) is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26 BAC. This modified BAC was used for Tg mouse production. To achieve high-level transgene expression, C1V1-EYFP is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region. R26 BAC::pCAG-LF-C1V1-EYFP line (RBRC06588), R26 BAC::pCAG-LoxP-C1V1-EYFP line (RBRC06589) (FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain), R26 BAC::pCAG-FRT-C1V1-EYFP line (RBRC06590) (LoxP-Stop-LoxP cassette was removed by crossing TNAP-Cre strain).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe SorianoUNGt(ROSA)26Sor

Gene symbolGene nameChr.Allele symbolAllele namePromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
beta-globin polyA signal (Rabbit), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signalUN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
C1V1 (Chlamydomonas reinhardtii, Volvox carteri)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, chicken beta-actin intron, rabbit beta-globin intron), Growth hormone polyA signal (Bovine), Neo (E. coli), phosphoglucokinase gene <PGK> promoter (mouse), beta-globin poly A signal (Rabbit), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP sites, Yeast FRT (flippase recombination target) sites, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), C1V1 (Chlamydomonas reinhardtii, Volvox carteri), Jellyfish EYFP, BAC containing mouse Rosa26 locus (RP23-401D9)
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (後日指定)In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the C1V1・ChR2(SSFO) (Dr. Karl Deisseroth, http://web.stanford.edu/group/dlab/optogenetics/request_dna.html).
DepositorItaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data