Strain Data Sheet
RBRC06587
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC06587 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6-Tg(Gt(ROSA)26Sor-ArchT/EGFP)1.2Imayo |
| Former Common name | R26 BAC::pCAG-FRT-ArchT-EGFP, B6-ROSA26/ArchT-GFP/Frt Tg |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Itaru Imayoshi, Institute for Virus Research, Kyoto University in 2012. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. |
| Strain description | ArchT-EGFP is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26 BAC. This modified BAC was used for Tg mouse production. To achieve high-level transgene expression, ArchT-EGFP is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region. R26 BAC::pCAG-LF-ArchT-EGFP line (RBRC06585), R26 BAC::pCAG-LoxP-ArchT-EGFP line (RBRC06586) (FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain), R26 BAC::pCAG-FRT-ArchT-EGFP line (RBRC06587) (LoxP-Stop-LoxP cassette was removed by crossing TNAP-Cre strain). |
| Colony maintenance | Carrier x Noncarrier [C57BL/6JJmsSlc] |
| References | |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| ArchT (Halorubrum) | UN | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||||
| beta-globin polyA signal (rabbit), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal | UN | ||||||
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | UN | EGFP | ||||
| Frt | yeast FRT (flippase recombination target) site | UN | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | UN | Frt | ||||
| GH | Growth hormone polyA (Bovine) | UN | GH | ||||
| Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano | UN | Gt(ROSA)26Sor | ||||
| WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | UN | WPRE | ||||
| loxP | phage P1 loxP | UN | loxP | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, chicken beta-actin intron, rabbit beta-globin intron), Growth hormone polyA signal (Bovine), Neo (E. coli), phosphoglucokinase gene <PGK> promoter (mouse), beta-globin poly A signal (Rabbit), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP site, Yeast FRT (flippase recombination target) sites, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), ArchT (Halorubrum), Jellyfish EGFP cDNA, BAC containing mouse Rosa26 locus (RP23-401D9) |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). |
| Depositor | Itaru Imayoshi (Kyoto University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |