Strain Data Sheet
RBRC06583
Strain Information | |
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| Image | |
| BRC No. | RBRC06583 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6-Tg(Gt(ROSA)26Sor-eNpHR3/EYFP)1Imayo |
| Former Common name | R26 BAC::pCAG-LF-eNpHR3-EYFP, B6-ROSA26/eNpHR3-YFP/LF Tg |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Itaru Imayoshi, Institute for Virus Research, Kyoto University in 2012. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. |
| Strain description | eNpHR3-EYFP is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26 BAC. This modified BAC was used for Tg mouse production. To achieve high-level transgene expression, eNpHR3-EYFP is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region. R26 BAC::pCAG-LF-eNpHR3-EYFP line (RBRC06583), R26 BAC::pCAG-LoxP-eNpHR3-EYFP line (RBRC06584) (FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain.). |
| Colony maintenance | Carrier x Noncarrier [C57BL/6JJmsSlc] |
| References | |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| beta-globin polyA signal (rabbit), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal | UN | ||||||
| EYFP | Enhanced Yellow Fluorescent Protein (Aequorea victoria) | UN | EYFP | ||||
| Frt | yeast FRT (flippase recombination target) site | UN | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | UN | Frt | ||||
| GH | Growth hormone polyA (Bovine) | UN | GH | ||||
| Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano | UN | Gt(ROSA)26Sor | ||||
| WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | UN | WPRE | ||||
| eNpHR3 | Natronomonas enhanced halorhodopsin 3 | UN | eNpHR3 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| loxP | phage P1 loxP | UN | loxP | ||||
| loxP | phage P1 loxP | UN | loxP | ||||
| neo | neomycin resistance gene (E. coli) | UN | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, chicken beta-actin intron, rabbit beta-globin intron), Bacteriophage P1 loxP sites, phosphoglucokinase gene <PGK> promoter (mouse), Neo (E. coli), Growth hormone polyA signal (Bovine), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Yeast FRT (flippase recombination target) sites, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), Natronomonas enhanced halorhodopsin 3 (eNpHR3), Jellyfish EYFP cDNA, BAC containing mouse Rosa26 locus (RP23-401D9) |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). |
| Depositor | Itaru Imayoshi (Kyoto University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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