Strain Data Sheet

RBRC06581

Strain Information

Image
BRC No.RBRC06581
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Dlx5/6-tTAad)2Imayo
Former Common nameDlx5/6-tTA-Ad Line2, B6-Dlx-tTAad Tg#2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi, Institute for Virus Research, Kyoto University in 2010. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptiontTA-Ad is expressed under the Dlx5/6 enhancer. Line 1 (RBRC06580), Line 2 (RBRC06581), Line 3 (RBRC06582).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signalUN
  • split hand-foot malformation 1 with sensorineural hearing loss(MedGEN)

  • split hand-foot malformation 1(MedGEN)
    • immunoglobulin gene heavy chain variable region (human)UN
  • split hand-foot malformation 1 with sensorineural hearing loss(MedGEN)

  • split hand-foot malformation 1(MedGEN)
    • HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA
  • split hand-foot malformation 1 with sensorineural hearing loss(MedGEN)

  • split hand-foot malformation 1(MedGEN)
    • HBBhuman beta-globinUNHBB
  • split hand-foot malformation 1 with sensorineural hearing loss(MedGEN)

  • split hand-foot malformation 1(MedGEN)
    • WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE
  • split hand-foot malformation 1 with sensorineural hearing loss(MedGEN)

  • split hand-foot malformation 1(MedGEN)
    • tTAadtTA-Advanced (synthetic)UNtTAadmouse Dlx5/6 enhancer
  • split hand-foot malformation 1 with sensorineural hearing loss(MedGEN)

  • split hand-foot malformation 1(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Dlx5/6 enhancer <m56i>, b-globin basal promoter (human),Chimeric intron <5'-donor site from the first intron of the human β-globin gene and the branch and 3'-acceptor site from the intron of an immunoglobulin gene heavy chain variable region (human), HA (hemagglutinin) tag sequence, tTA-Advanced (synthetic), Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), SV40 PolyA signal
    Research applicationNeurobiology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
    DepositorItaru Imayoshi (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data