Strain Data Sheet
RBRC06579
Information1 | |
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| Image | |
| BRC No. | RBRC06579 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6J-Tg(Thy1-G-CaMP7,-DsRed2)492Bsi |
| Former Common name | Thy1-G-CaMP7 Tg, B6-Thy1-GCMP7/DsRed, Tg#492 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Masamichi Ohkura and Junichi Nakai, Saitama University, Masaaki Sato and Yasunori Hayashi, RIKEN Brain Institute in 2011. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. |
| Strain description | Thy1-G-CaMP7/DsRed2 transgenic mice. These transgenic mice express G-CAMP7 and DsRed2 under the control of the Thy1 promoter. |
| Colony maintenance | Carrier x Noncarrier [or Crossing to C57BL/6JJmsSlc] |
| References | PLoS One, 7, e51286, (2012). 23240011 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter DsRed2Red Fluorescent Protein (Discosoma sp.)UNDsRed2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GCaMP7RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP7mouse Thy1.2 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter T2AThosea asigna virus 2A peptide (insect)UNT2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signalUN |
Information2 | |
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| Donor DNA | GCaMP7(RSET tag, Chicken myosin light chain kinase (M13) cDNA, Aequorea Victoria GFP cDNA, Rat calmodulin cDNA), Thosea asigna virus 2A peptide (T2A), Discosoma sp. DsRed2 cDNA, mouse Thy1.2 genomic DNA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 7, e51286, (2012).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Yasunori Hayashi (RIKEN) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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