Strain Data Sheet

RBRC06579

Information1

Image
BRC No.RBRC06579
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Thy1-G-CaMP7,-DsRed2)492Bsi
Former Common nameThy1-G-CaMP7 Tg, B6-Thy1-GCMP7/DsRed, Tg#492
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masamichi Ohkura and Junichi Nakai, Saitama University, Masaaki Sato and Yasunori Hayashi, RIKEN Brain Institute in 2011. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain descriptionThy1-G-CaMP7/DsRed2 transgenic mice. These transgenic mice express G-CAMP7 and DsRed2 under the control of the Thy1 promoter.
Colony maintenanceCarrier x Noncarrier [or Crossing to C57BL/6JJmsSlc]
ReferencesPLoS One, 7, e51286, (2012). 23240011

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DsRed2Red Fluorescent Protein (Discosoma sp.)UNDsRed2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GCaMP7RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP7mouse Thy1.2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)UNT2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Information2

Donor DNAGCaMP7(RSET tag, Chicken myosin light chain kinase (M13) cDNA, Aequorea Victoria GFP cDNA, Rat calmodulin cDNA), Thosea asigna virus 2A peptide (T2A), Discosoma sp. DsRed2 cDNA, mouse Thy1.2 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 7, e51286, (2012).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYasunori Hayashi (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data