Strain Data Sheet

RBRC06577

Strain Information

Image
BRC No.RBRC06577
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Reck<tm2.1Noda>
Former Common nameRECK floxed, B6-Reck floxed
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Mako Yamamoto, Kyoto University Graduate School of Medicine, Shigeyoshi Itohara, RIKEN Brain Research Institute. E14 ES cells were used. A neo cassette was removed by crossing with EIIa-cre Tg mice. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionReck gene floxed mice. Exon 1 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
References
The transformation suppressor gene Reck is required for postaxial patterning in mouse forelimbs.
Yamamoto M, Matsuzaki T, Takahashi R, Adachi E, Maeda Y, Yamaguchi S, Kitayama H, Echizenya M, Morioka Y, Alexander D B, Yagi T, Itohara S, Nakamura T, Akiyama H, Noda M
Biology Open, 1, 458-466 (2012). 23213437

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Reckreversion-inducing-cysteine-rich protein with kazal motifs4Recktargeted mutation 2.1, Makoto Noda
loxPphage P1 loxP4loxP
loxPphage P1 loxP4loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, mouse Reck genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biology Open, 1, 458-466 (2012).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT shall reach agreement on terms and conditions of use of it with DEPOSITOR and shall obtain a prior written consent from the DEPOSITOR. The RECIPIENT shall contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMakoto Noda (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data