Strain Data Sheet

RBRC06571

Strain Information

Image
BRC No.RBRC06571
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(RARE-Hspa1b/lacZ)12Jrt
Former Common nameRARE-LacZ, RARE-lacZ Tg#12
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Janet Rossant, Mount Sinai Hospital. Maintained by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2012. The transgene was injected into the pronuclei of CD-1 fertilized eggs. Mixed background.
Strain descriptionRARE (retinoic acid responsive element)-lacZ reporter transgenic mice. Tg mice are viable and fertile.
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:B6C3F1]
ReferencesGenes Dev. 5(8). 1333-1344 (1991). 1907940

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rarbretinoic acid receptor, betaUNRarb

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)UNmouse Hspa1b promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Ordering Information

Donor DNAmouse Rarb retinoic acid responsive element genomic DNA, mouse Hspa1b promoter, E. coli LacZ, SV40 polyA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 5(8). 1333-1344 (1991).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Otomo K, Omura T, Nozawa Y, Edwards SJ, Sato Y, Saito Y, Yagishita S, Uchida H, Watakabe Y, Naitou K, Yanai R, Sahara N, Takagi S, Katayama R, Iwata Y, Shiokawa T, Hayakawa Y, Otsuka K, Watanabe-Takano H, Haneda Y, Fukuhara S, Fujiwara M, Nii T, Meno C, Takeshita N, Yashiro K, Rosales Rocabado JM, Kaku M, Yamada T, Oishi Y, Koike H, Cheng Y, Sekine K, Koga JI, Sugiyama K, Kimura K, Karube F, Kim H, Manabe I, Nemoto T, Tainaka K, Hamada A, Brismar H, Susaki EA.
descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.
Nat Commun 15(1) 4941(2024) 38866781