RBRC06563

Information1

Image
BRC No.RBRC06563
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rhof<tm1.2Dash>
Former Common nameRhoF KO, B6-Rhof KO
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by Mayuko Kishimoto and Tadashi Matsushita, Nagoya University Graduate School of Medicine. EGR-G01 ES cells were used.
Strain descriptionRhoF gene knockout mice. Exon 2 was deleted. Homozygous mutant mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or C57BL/6NCrSlc]
ReferencesNagoya J. Med. Sci., 76, 293-305 (2014).

Health Report

Examination Date / Room / Rack2017/06/19Room:3-CRack:F
2017/04/17Room:3-CRack:F
2017/02/20Room:3-CRack:F
2016/12/19Room:3-CRack:F
2016/10/17Room:3-CRack:F
2016/08/22Room:3-CRack:F
2016/06/20Room:3-CRack:F
2016/04/18Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rhofras homolog gene family, member f5Rhof<tm1.2Dash>targeted mutation 1.2, Tadashi Matsushita

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Information2

Donor DNAphage P1 loxP site, mouse Rhof genomic DNA,[CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 Cre recombinase]
Research applicationCre/loxP system
Hematological Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nagoya J. Med. Sci. , 76, 293-305 (2014). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTadashi Matsushita (Nagoya University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data