RBRC06549

Information1

Image
BRC No.RBRC06549
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nodal<tm3.1Hmd>
Former Common nameNodal RAREn-flox, B6-Nodal/RAREn floxed
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Masayuki Uehara and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2009. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. A frt flanked PGK-neo cassette was removed by crossing with CAG-FLPe mice (B6/129 background). C57BL/6 and 129 mixed background.
Strain descriptionNodal gene REREn floxed mice. REREn in intron 1 of the Nodal gene was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nodalnodal10Nodal<tm3.1Hmd>targeted mutation 3.1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP10loxP

Information2

Donor DNAphage P1 loxP site, yeast FRT (flipase recombination target) site, mouse Nodal genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data