Strain Data Sheet

RBRC06542

Strain Information

Image
BRC No.RBRC06542
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Lrrc6<tm1.2Hmd>
Former Common nameLrrc6 null, B6-Lrrc6 KO
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Katsura Minegishi and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. Flanked Exon 1 was removed by crossing with CAG-Cre mice (B6/129 background). C57BL/6 and 129 mixed background.
Strain descriptionExon 1 of Lrrc6 gene was removed.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Transport of the outer dynein arm complex to cilia requires a cytoplasmic protein Lrrc6.
Inaba Y, Shinohara K, Botilde Y, Nabeshima R, Takaoka K, Ajima R, Lamri L, Takeda H, Saga Y, Nakamura T, Hamada H
Genes Cells, 21, 728-739 (2016). 27353389

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Lrrc6leucine rich repeat containing 6 (testis)15Lrrc6targeted mutation 1.2, Hiroshi Hamada
  • primary ciliary dyskinesia 19(DisGeNET, MedGEN)
    • loxPphage P1 loxP15loxP
  • primary ciliary dyskinesia 19(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, mouse Lrrc6 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells, 21, 728-739 (2016).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data