Strain Data Sheet
RBRC06539
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC06539 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Dchs2<tm1.1Hmd> |
| Former Common name | Dachsous2 flox, B6-Dchs2 floxed |
| H-2 Haplotype | |
| ES Cell line | G4 [(129S6/SvEvTac x C57BL/6NCr)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Katsura Minegishi and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2012. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. A frt flanked PGK-neo cassette was removed by crossing with CAG-FLPe mice (B6/129 background). C57BL/6 and 129 mixed background. |
| Strain description | Dchs2 (dachsous 2) gene floxed mice. Exon 29 was flanked by loxP sites. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
| References | Planar cell polarity-dependent asymmetric organization of microtubules for polarized positioning of the basal body in node cells. Xiaorei Sai, Yayoi Ikawa, Hiromi Nishimura, Katsutoshi Mizuno, Eriko Kajikawa, Takanobu A Katoh, Toshiya Kimura, Hidetaka Shiratori, Katsuyoshi Takaoka, Hiroshi Hamada, Katsura Minegishi Development, 149(9):dev200315 (2022). doi: 10.1242/dev.200315 35420656 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/04/22Room:3-CRack:J |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Dchs2 | dachsous cadherin related 2 | 3 | Dchs2 | targeted mutation 1.1, Hiroshi Hamada | |||
| Frt | yeast FRT (flippase recombination target) site | 3 | Frt | ||||
| loxP | phage P1 loxP | 3 | loxP | ||||
| loxP | phage P1 loxP | 3 | loxP | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Dchs2 genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 149(9):dev200315 (2022). |
| Depositor | Hiroshi Hamada (RIKEN CDB) |
| Strain Status |  Live mice |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
|---|
| No Data |