Strain Data Sheet

RBRC06536

Information1

Image
BRC No.RBRC06536
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Uhrf1<tm2.1Hko>
Former Common nameNP95 cond ver.2, 6-Uhrf1 floxed
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN Center for Integrative Medical Sciences. M1 ES cell were used to generate the mutant mice.
Strain descriptionUhrf1 gene floxed mice. Gene targeting for the Np95 (also known as Uhrf1, ICBP90) allele was done by flanking exons 4 and 5 with Cre-loxP sequences. Conditional deletion of Np95 (exons 4,5) can be achieved by crossing the mice with specific cre deleters such as CreERT2. The Np95 protein is an epigenetic reader and writer molecule, involved in maintenance DNA methylation (Sharif et al., 2007), recognition of H3K9 di/tri methylation (Rothbart et al., 2012) and ubiquitination of histone H3 (Nishiyama et al., 2013). The constitutive null (Np95-KO) mice are embryonically lethal post 9.5 dpc. Ablation of Np95 causes genomewide loss of DNA methylation marks (Sharif et al., 2007). This conditional allele would be useful to study the roles of Np95 in DNA methylation and regulation of other epigenetic modifications such as H3K9 methylation and H3 ubiquitination, by combining with tissue specific Cre deleters
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesNature, 502, 249-253 (2013). 24013172
Nature, 450, 908-912 (2007). 17994007
Nat Immunol. 15, 571-579 (2014). 24777532

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Uhrf1ubiquitin-like, containing PHD and RING finger domains, 117Uhrf1<tm2.1Hko>targeted mutation 2.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site17Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Uhrf1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsRECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month May 2015
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data