Strain Data Sheet
RBRC06536
Strain Information | |
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| Image | |
| BRC No. | RBRC06536 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Uhrf1<tm2.1Hko> |
| Former Common name | NP95 cond ver.2, 6-Uhrf1 floxed |
| H-2 Haplotype | |
| ES Cell line | M1 [(C57BL/6 x 129)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Haruhiko Koseki, RIKEN Center for Integrative Medical Sciences. M1 ES cell were used to generate the mutant mice. |
| Strain description | Uhrf1 gene floxed mice. Gene targeting for the Np95 (also known as Uhrf1, ICBP90) allele was done by flanking exons 4 and 5 with Cre-loxP sequences. Conditional deletion of Np95 (exons 4,5) can be achieved by crossing the mice with specific cre deleters such as CreERT2. The Np95 protein is an epigenetic reader and writer molecule, involved in maintenance DNA methylation (Sharif et al., 2007), recognition of H3K9 di/tri methylation (Rothbart et al., 2012) and ubiquitination of histone H3 (Nishiyama et al., 2013). The constitutive null (Np95-KO) mice are embryonically lethal post 9.5 dpc. Ablation of Np95 causes genomewide loss of DNA methylation marks (Sharif et al., 2007). This conditional allele would be useful to study the roles of Np95 in DNA methylation and regulation of other epigenetic modifications such as H3K9 methylation and H3 ubiquitination, by combining with tissue specific Cre deleters |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
| References | Uhrf1-dependent H3K23 ubiquitylation couples maintenance DNA methylation and replication. Nishiyama A, Yamaguchi L, Sharif J, Johmura Y, Kawamura T, Nakanishi K, Shimamura S, Arita K, Kodama T, Ishikawa F, Koseki H, Nakanishi M Nature, 502, 249-253 (2013). 24013172The epigenetic regulator Uhrf1 facilitates the proliferation and maturation of colonic regulatory T cells. Obata Y, Furusawa Y, Endo T A, Sharif J, Takahashi D, Atarashi K, Nakayama M, Onawa S, Fujimura Y, Takahashi M, Ikawa T, Otsubo T, Kawamura Y I, Dohi T, Tajima S, Masumoto H, Ohara O, Honda K, Hori S, Ohno H, Koseki H, Hase K Nat Immunol. 15, 571-579 (2014). 24777532The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Sharif J, Muto M, Takebayashi S, Suetake I, Iwamatsu A, Endo T A, Shinga J, Mizutani-Koseki Y, Toyoda T, Okamura K, Tajima S, Mitsuya K, Okano M, Koseki H Nature, 450, 908-912 (2007). 17994007 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 17 | Frt | ||||
| Uhrf1 | ubiquitin-like, containing PHD and RING finger domains, 1 | 17 | Uhrf1 | targeted mutation 2.1, Haruhiko Koseki | |||
| loxP | phage P1 loxP | 17 | loxP | ||||
| loxP | phage P1 loxP | 17 | loxP | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | more 5 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Uhrf1 genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Haruhiko Koseki (RIKEN) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month May 2015 |
BRC mice in Publications |
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| No Data |