Strain Data Sheet

RBRC06532

Strain Information

Image
BRC No.RBRC06532
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Jmjd1c<tm1Mtac>
Former Common nameJmjd1c KO, B6-Jmjd1c KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Matoko Tachibana, Institute for Virus Research, Kyoto Uiniversity in 2008. TT2 ES cells were used to generate the mutant mice. C57BL/6 semicongenic.
Strain descriptionJmjd1c gene knockout mice. Exons 24 to 57 was replaced with an IRES-neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
JMJD1C, a JmjC domain-containing protein, is required for long-term maintenance of male germ cells in mice.
Kuroki S, Akiyoshi M, Tokura M, Miyachi H, Nakai Y, Kimura H, Shinkai Y, Tachibana M
Biology of reproduction 89(4):93,1-9 (2013). 24006281

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signal10
IRESinternal ribosomal entry site (EMCV)10
Jmjd1cjumonji domain containing 1C10Jmjd1ctargeted mutation 1, Makoto Tachibana
neoneomycin resistance gene (Streptomyces fradiase)10

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal male germ cell apoptosis(MP:0008280)

  • abnormal seminiferous tubule epithelium morphology(MP:0011750)

  • abnormal spermatogonia morphology(MP:0006378)

  • decreased male germ cell number(MP:0004901)

  • decreased testis weight(MP:0004852)
    • more 5 phenotypes
  • early reproductive senescence(MP:0008995)

  • oligozoospermia(MP:0002687)

  • reproductive system phenotype(MP:0005389)

  • seminiferous tubule degeneration(MP:0001154)

  • small testis(MP:0001147)
    • Detailed phenotype data

    Ordering Information

    Donor DNAStreptomyces fradiase neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Simian virus 40 polyA additional signal, mouse jumonji domain containing 1C genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biology of reproduction, 89(4):93,1-9 (2013).
    DepositorMakoto Tachibana (Tokushima University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data