Strain Data Sheet

RBRC06523

Strain Information

Image
BRC No.RBRC06523
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Cthrc1<tm1.2Suna>
Former Common nameCthrc1 sKO, B6-Cthrc1 KO
H-2 Haplotype
ES Cell lineiTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Sunao Takeshita, National Center for Geriatrics and Gerontology. (C57BL/6NTac x 129S6/SvEvTac)F1 derived ES cells were used to generate the mutant mice. C57BL/6 background. Floxed mice (RBRC06522): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice. KO mice (RBRC06523): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice, and then the exon 2 was removed by crossing with CAG-Cre mice.
Strain descriptionCthrc1 gene floxed mice (RBRC06522)(Exon 2 was flanked by loxP sites). Cthrc1 gene KO mice (RBRC06523)(Exon 2 was deleted).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Osteoclast-secreted CTHRC1 in the coupling of bone resorption to formation.
Takeshita S, Fumoto T, Matsuoka K, Park K A, Aburatani H, Kato S, Ito M, Ikeda K
J Clin Invest., 123, 3914-3924 (2013). 23908115

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cthrc1collagen triple helix repeat containing 115Cthrc1targeted mutation 1.2, Sunao Takeshita
  • Barrett esophagus(MedGEN)
    • Frtyeast FRT (flippase recombination target) site15Frt
  • Barrett esophagus(MedGEN)
    • loxPphage P1 loxP15loxP
  • Barrett esophagus(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased bone mass(MP:0004016)

  • decreased bone trabecula number(MP:0010869)

  • decreased trabecular bone thickness(MP:0009346)

  • osteoporosis(MP:0000066)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Cthrc1 gene, yeast FRT (flipase recombination target) site, P1 phage loxP site
    Research applicationCancer Research
    Cre/loxP system
    FLP/frt system
    Hematological Research
    Metabolism Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Clin. Invest., 123, 3914-3924 (2013).
    DepositorKyoji Ikeda (National Center for Geriatrics and Gerontology)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data