Strain Data Sheet

RBRC06520

Information1

Image
BRC No.RBRC06520
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Crh<tm2(cre)Ksak>
Former Common nameCRF-iCre, B6-Crh/cre KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Keiichi Itoi, Graduate School of Information Sciences, Tohoku University and Kenji Sakimura, Brain Research Institute, Niigata University in 2011. RENKA ES cells were used to generate the mutant mice.
Strain descriptionCrh (CRF, Corticotropin releasing factor) gene cre knock-in mice. An iCre-frt-flanked PGK-neo cassette was inserted at the translation initiation site of the CRF gene.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
ReferencesEndocrinology. 155. 4054-4060 (2014). 25057791

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Crhcorticotropin releasing hormone3Crh<tm2(cre)Ksak>targeted mutation 2, Kenji Sakimura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
iCrephage P1 codon improved Cre (iCre)3iCre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3mouse Phosphoglycerate kinase promoter (PGK promoter), gb2 promoter

Information2

Donor DNAphage P1 improved Cre recombinase DNA, yeast FRT (flipase recombination target) sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter(synthetic), E. coli Neomycin resistance gene, mouse Crh genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Endocrinology. 2014 en20141182.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKeiichi Itoi (Tohoku University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data