Strain Data Sheet

RBRC06517

Information1

Image
BRC No.RBRC06517
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm10(PITT)Maoh>
Former Common nameTOKMO-3, B6-ROSA26/PITT KI
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain developmentDeveloped by Masato Ohtsuka, Tokai University School of Medicine in 2014. EGR101 ES cells were used to generate the mutant mice.
Strain descriptionSA-F14-attP-JT15-eGFP-T2A-neo-hOCT4 ZFN target site-pA-FRT-L-lox2272-F15 ROSA26 knock-in mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6N]
ReferencesBMC Genomics 2015, 16:274 25887549

Health Report

Examination Date / Room / Rack2021/08/16Room:3-CRack:D
2021/05/17Room:3-CRack:D
2021/02/15Room:3-CRack:D
2018/04/16Room:3-CRack:F
2018/02/19Room:3-CRack:F
2017/12/18Room:3-CRack:F
2017/10/16Room:3-CRack:F
2017/08/21Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm10(PITT)Maoh>targeted mutation 10, Masato Ohtsuka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F14yeast FRT (flippase recombination target) site (synthetic) variant (F14)6F14

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F15yeast FRT (flippase recombination target) site (synthetic) variant (F15)6F15

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FRT-Lyeast FRT (flippase recombination target) site, variant (FRT-L)6FRT-L

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
JT15phage P1 loxP variant (JT15)6JT15

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor (adenovirus)6SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)6T2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
attStreptomyces lividans attachment site6att

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
attactinophage phi C31 attachment site6att

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox2272mutant lox sequence6lox2272

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal6

Information2

Donor DNAmouse Gt(ROSA)26Sor, adenovirus SA, yeast FRT variant (F14), Streptomyces lividans and actinophage phi C31 attP, phage P1 loxP variant (JT15), jellyfish eGFP cDNA, insect Thosea asigna virus T2A peptide, E. coli neo, human OCT4 cDNA, Simian virus 40 (SV40) polyA, yeast FRT variant (FRT-L), phage P1 loxP variant (lox2272), yeast FRT variant (F15)
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定。 (will be announced).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in the first publication of the results obtained from the use of the BIOLOGICAL RESOURCE.
DepositorMasato Ohtsuka (Tokai University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Mouse of the Month Sep 2015
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data