Strain Data Sheet

RBRC06514

Strain Information

Image
BRC No.RBRC06514
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Dock5<Gt(AN0268)Wtsi>/Shigm
Former Common nameDock5 KO, Dock5 Gt
H-2 Haplotype
ES Cell lineE14TG2aIV [129P2/OlaHsd]
Background strain
Appearanceagouti
Strain developmentDeveloped by Jean-Franc Francois Cote, Montreal University. Maintained by Hiroshi Hiai, Shiga Medical Center for Adults. 129, BALB/c and other strain? mixed background.
Strain descriptionDock5 gene trap mice. The pGTOlxr gene trap vector containing a beta-geo cassette was inserted into the intron 29 of Dock5 gene.
Colony maintenanceHomozygote x Homozygote
References
The atypical Rac activator Dock180 (Dock1) regulates myoblast fusion in vivo.
Laurin M, Fradet N, Blangy A, Hall A, Vuori K, Cote J F
Proc. Natl. Acad. Sci. USA, 105. 15446-15451 (2008). 18820033

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA14
Dock5dedicator of cytokinesis 514Dock5gene trap AN0268, Wellcome Trust Sanger Institute
Frtyeast FRT (flippase recombination target) site14Frt
Frtyeast FRT (flippase recombination target) site14Frt
lacZbeta-galactosidase (E. coli)14
lox71mutant lox sequence14lox71
loxPphage P1 loxP14loxP
neoneomycin resistance gene (E. coli)14

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • mortality/aging(MP:0010768)

  • muscle phenotype(MP:0005369)
    • Detailed phenotype data

    Ordering Information

    Donor DNApGT0Lxr gene trap cassette (1.5 kb of mouse En2 intron 1, mouse En2 splice acceptor, yeast FRT (flipase recombination target) sites, phage P1 lox71 site, phage P1 loxP site, E.coli lacZ, E.coli neo, SV40 polyA, pUC backbone)
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 105, 15446-15451 (2008).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorKazuo Kinoshita (Shiga Medical Center Research Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data