RBRC06514

Information1

Image
BRC No.RBRC06514
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Dock5<Gt(AN0268)Wtsi>/Shigm
Former Common nameDock5 KO, Dock5 Gt
H-2 Haplotype
ES Cell lineE14TG2aIV [129P2/OlaHsd]
Background strain
Appearanceagouti
Strain developmentDeveloped by Jean-Franc Francois Cote, Montreal University. Maintained by Hiroshi Hiai, Shiga Medical Center for Adults. 129, BALB/c and other strain? mixed background.
Strain descriptionDock5 gene trap mice. The pGTOlxr gene trap vector containing a beta-geo cassette was inserted into the intron 29 of Dock5 gene.
Colony maintenanceHomozygote x Homozygote
ReferencesProc. Natl. Acad. Sci. USA, 105. 15446-15451 (2008). 18820033

Health Report

Examination Date / Room / Rack2020/06/15Room:3-ARack:G
2020/06/01Room:3-ARack:G
2020/02/17Room:3-ARack:G
2019/12/16Room:3-ARack:G
2019/10/21Room:3-ARack:G
2019/08/26Room:3-ARack:G
2019/06/17Room:3-ARack:G
2019/04/15Room:3-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dock5Dedicator of cytokinesis 514Dock5<Gt(AN0268)Wtsi>gene trap AN0268, Wellcome Trust Sanger Institute

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)14

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox71mutant lox sequence14lox71

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)14

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA14

Information2

Donor DNApGT0Lxr gene trap cassette (1.5 kb of mouse En2 intron 1, mouse En2 splice acceptor, yeast FRT (flipase recombination target) sites, phage P1 lox71 site, phage P1 loxP site, E.coli lacZ, E.coli neo, SV40 polyA, pUC backbone)
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 105, 15446-15451 (2008).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorKazuo Kinoshita (Shiga Medical Center Research Institute)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (within 1 month)
Additional Info.

BRC mice in Publications

No Data