Strain Data Sheet

RBRC06513

Strain Information

Image
BRC No.RBRC06513
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Pax6<tm2(iCre)Fmur>
Former Common namePax6F2AiCre, B6-Pax6/F2Acre KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrl
Appearance
Strain developmentDeveloped by Fujio Murakami, Graduate School of Frontier Biosciences, Osaka University in 2014. RENKA ES cells were used to generate the mutant mice.
Strain descriptionPax6 hgene Cre knock-in mice. F2A, codon improved Cre-polyA and frt-flanked pgk/gb2-neo cassettes were inserted into the exon 13.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signal2
  • aniridia 1(MedGEN)

  • aniridia(DisGeNET)

  • autosomal dominant keratitis(MedGEN)
    • more 5 Diseases
  • coloboma of optic nerve(MedGEN)

  • coloboma, ocular, autosomal dominant(MedGEN)

  • foveal hypoplasia 1(MedGEN)

  • Peters anomaly(MedGEN)

  • WAGR syndrome(DisGeNET, MedGEN)
    • F2Afoot-and-mouth disease virus (FMD) 2A peptide2F2A
  • aniridia 1(MedGEN)

  • aniridia(DisGeNET)

  • autosomal dominant keratitis(MedGEN)
    • more 5 Diseases
  • coloboma of optic nerve(MedGEN)

  • coloboma, ocular, autosomal dominant(MedGEN)

  • foveal hypoplasia 1(MedGEN)

  • Peters anomaly(MedGEN)

  • WAGR syndrome(DisGeNET, MedGEN)
    • Frtyeast FRT (flippase recombination target) site2Frt
  • aniridia 1(MedGEN)

  • aniridia(DisGeNET)

  • autosomal dominant keratitis(MedGEN)
    • more 5 Diseases
  • coloboma of optic nerve(MedGEN)

  • coloboma, ocular, autosomal dominant(MedGEN)

  • foveal hypoplasia 1(MedGEN)

  • Peters anomaly(MedGEN)

  • WAGR syndrome(DisGeNET, MedGEN)
    • Frtyeast FRT (flippase recombination target) site2Frt
  • aniridia 1(MedGEN)

  • aniridia(DisGeNET)

  • autosomal dominant keratitis(MedGEN)
    • more 5 Diseases
  • coloboma of optic nerve(MedGEN)

  • coloboma, ocular, autosomal dominant(MedGEN)

  • foveal hypoplasia 1(MedGEN)

  • Peters anomaly(MedGEN)

  • WAGR syndrome(DisGeNET, MedGEN)
    • Pax6paired box 62Pax6targeted mutation 2, Fujio Murakami
  • aniridia 1(MedGEN)

  • aniridia(DisGeNET)

  • autosomal dominant keratitis(MedGEN)
    • more 5 Diseases
  • coloboma of optic nerve(MedGEN)

  • coloboma, ocular, autosomal dominant(MedGEN)

  • foveal hypoplasia 1(MedGEN)

  • Peters anomaly(MedGEN)

  • WAGR syndrome(DisGeNET, MedGEN)
    • iCrephage P1 codon improved Cre (iCre)2iCre
  • aniridia 1(MedGEN)

  • aniridia(DisGeNET)

  • autosomal dominant keratitis(MedGEN)
    • more 5 Diseases
  • coloboma of optic nerve(MedGEN)

  • coloboma, ocular, autosomal dominant(MedGEN)

  • foveal hypoplasia 1(MedGEN)

  • Peters anomaly(MedGEN)

  • WAGR syndrome(DisGeNET, MedGEN)
    • neoneomycin resistance gene (E. coli)2mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter
  • aniridia 1(MedGEN)

  • aniridia(DisGeNET)

  • autosomal dominant keratitis(MedGEN)
    • more 5 Diseases
  • coloboma of optic nerve(MedGEN)

  • coloboma, ocular, autosomal dominant(MedGEN)

  • foveal hypoplasia 1(MedGEN)

  • Peters anomaly(MedGEN)

  • WAGR syndrome(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAFoot-and-mouth disease virus (FMDV derived) 2A peptide (F2A), Bacteriophage P1 codon improved Cre (iCre), SV40 PolyA signal, Saccharomyces cerevisiae Flp recombinase target (FRT) sites, Mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli Neomycin resistance gene, mouse Pax6 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR (Dr. Fujio Murakami, Osaka University) and DEVELOPER (Dr. Kenji Sakimura, Nigata University) is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorFujio Murakami (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data