RBRC06513

Information1

Image
BRC No.RBRC06513
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Pax6<tm2(iCre)Fmur>
Former Common namePax6F2AiCre, B6-Pax6/F2Acre KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrl
Appearance
Strain developmentDeveloped by Fujio Murakami, Graduate School of Frontier Biosciences, Osaka University in 2014. RENKA ES cells were used to generate the mutant mice.
Strain descriptionPax6 hgene Cre knock-in mice. F2A, codon improved Cre-polyA and frt-flanked pgk/gb2-neo cassettes were inserted into the exon 13.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pax6paired box 62Pax6<tm2(iCre)Fmur>targeted mutation 2, Fujio Murakami

Gene symbolGene nameChr.Allele symbolAllele namePromoter
F2AFoot-and-Mouth Disease Virus (FMDV) 2A peptide2F2A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
iCrephage P1 codon improved Cre (iCre)2iCre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2mouse Phosphoglycerate kinase promoter (PGK promoter), gb2 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 PolyA signal2

Information2

Donor DNAhuman Furin protein cDNA, Foot and mouse virus 2A sequence, Bacteriophage P1 codon improved Cre (iCre), SV40 PolyA signal, Saccharomyces cerevisiae Flp recombinase target (FRT) sites, Mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli Neomycin resistance gene, mouse Pax6 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定。(will be announced).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR (Dr. Fujio Murakami, Osaka University) and DEVELOPER (Dr. Kenji Sakimura, Nigata University) is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorFujio Murakami (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data