RBRC06511

Information1

Image
BRC No.RBRC06511
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Gt(ROSA)26Sor<tm1(Fucci2aR)Jkn>
Former Common nameR26Fucci2aR(RM185R), B6-ROSA26/Fucci2aR KI
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Richard Mort, MRC IGMM, University of Edinburgh in 2014. The mutant mice were corssed to C57BL/6. C57BL/6 mixed background.
Strain descriptionFucci2aR ROSA26 knock-in mice. Targeted insertion of CAG-floxNeopA-Fucci2aR-pA cassette into the mouse Rosa26 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J]
ReferencesCell Cycle, 13, 2681-2696 (2014). 25486356

Health Report

Examination Date / Room / Rack2020/06/01Room:3-CRack:K
2020/02/17Room:3-CRack:K
2019/12/16Room:3-CRack:K
2019/12/03Room:3-CRack:K
2019/10/21Room:3-CRack:K
2019/08/26Room:3-CRack:K
2019/06/17Room:3-CRack:K
2019/06/17Room:3-CRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Fucci2aR)Jkn>targeted mutation 1, Ian Jackson

Gene symbolGene nameChr.Allele symbolAllele namePromoter
CDT1chromatin licensing and DNA replication factor (human)6CDT1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GMNNgeminin (human)6GMNN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
T2AThosea asigna virus 2A peptide (insect)6T2A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mVenusyellow fluorescent protein (Aequorea victoria) A206K6mVenus

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
polyA6

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), P1 phage loxP sites, E. coli neo, polyA, human CDT1 cDNA, Discosoma sp. mCherry cDNA, Thosea asigna virus 2A peptide, Jellyfish variant of enhanced yellow fluorescent protein (mVenus) cDNA, human GMNN cDNA, mouse Rosa26 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and Conditions1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature(s) designated by the DEPOSITOR is requested. Cell Cycle 13:17, 2681-2696, 2014.
3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
5) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorRichard Mort (The University of Edinburgh)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (within 1 month)
Additional Info.UCSD MTA
Genotyping protocol -PCR-

BRC mice in Publications

No Data