RBRC06510

Information1

Image
BRC No.RBRC06510
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(tetO-G-CaMP7,-DsRed2)572Bsi
Former Common nameTRE-G-CaMP7, B6-tetO-GCMP7/DsRed Tg#572
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yasunori Hayashi, RIKEN Brain Institute. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain descriptionTRE-C-CaMP7/DsRed2 transgenic mice. These transgenic mice express G-CAMP7 and DsRed2 under the control of the TRE (tetracycline response element) promoter.
Colony maintenanceCarrier x Noncarrier [or Crossing to C57BL/6JJmsSlc]
ReferencesPLoS One, 7, e51286, (2012). 23240011
PLoS One, 10(5): e0125354 (2015). 25946002

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
DsRed2Red Fluorescent Protein (Discosoma sp.)UNDsRed2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GCaMP7RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP7E. coli TRE promoter (tetracycline responsive promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
T2AThosea asigna virus 2A peptide (insect)UNT2A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNAE. coli TRE promoter (tetracycline responsive promoter), GCaMP7(RSET tag, Chicken myosin light chain kinase (M13) cDNA, Aequorea Victoria GFP cDNA, Rat Calmodulin cDNA), Thosea asigna virus 2A peptide, Discosoma sp. DsRed2 cDNA, SV40 polyA signal
Research applicationFluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 10(5): e0125354, 2015. Sato et al. Generation and imaging of transgenic mice that express G-CaMP7 under a tetracycline response element. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYasunori Hayashi (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Mouse of the Month Jun 2015

BRC mice in Publications

No Data