RBRC06505

Information1

Image
BRC No.RBRC06505
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(CAG-cat,-3xFLAG/Dazl)3
Former Common nameCAG-CAT-3FLAG・Dazl#3, ICR-CAG-FLAG/Dazl Tg#3
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Atsushi Suzuki, Faculty of Engineering, Yokohama National University in 2012. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. ICR mixed background.
Strain description3xFLAG-DAZL conditional transgenic mice. Line 3 (RBRC06505)(main line), Line 4 (RBRC06506).
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:MCH(ICR)]
ReferencesProc. Natl. Acad. Sci. USA, 92, 160-164 (1995). 7816809

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dazldeleted in azoospermia-likeUNDazlCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
FLAGFLAG tag (synthetic)UNFLAG

Gene symbolGene nameChr.Allele symbolAllele namePromoter
catE. coli chloramphenicol acetyltransferase (CAT)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), E. coli chloramphenicol acetyltransferase, phage P1 loxP sites, 3xFLAG tag sequence, Mouse Daz-like gene cDNA, polyA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAtsushi Suzuki (Yokohama National University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data