Strain Data Sheet

RBRC06505

Strain Information

Image
BRC No.RBRC06505
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(CAG-cat,-3xFLAG/Dazl)3
Former Common nameCAG-CAT-3FLAG・Dazl#3, ICR-CAG-FLAG/Dazl Tg#3
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Atsushi Suzuki, Faculty of Engineering, Yokohama National University in 2012. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. ICR mixed background.
Strain description3xFLAG-DAZL conditional transgenic mice. Line 3 (RBRC06505)(main line), Line 4 (RBRC06506).
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:MCH(ICR)]
References
Site-specific recombination of a transgene in fertilized eggs by transient expression of Cre recombinase.
Araki K, Araki M, Miyazaki J, Vassalli P
Proc. Natl. Acad. Sci. USA, 92, 160-164 (1995). 7816809

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dazldeleted in azoospermia-likeUNDazlCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
FLAGFLAG tag (synthetic)UNFLAG
catE. coli chloramphenicol acetyltransferase (CAT)UN
loxPphage P1 loxPUNloxP
loxPphage P1 loxPUNloxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), E. coli chloramphenicol acetyltransferase, phage P1 loxP sites, 3xFLAG tag sequence, Mouse Daz-like gene cDNA, polyA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorAtsushi Suzuki (Yokohama National University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data