Strain Data Sheet

RBRC06504

Strain Information

Image
BRC No.RBRC06504
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Lamc1<tm2.1Ksek>
Former Common nameLamc1 EQ conditional KI, B6-Lamc1/EQ floxed
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kiyotoshi Sekiguchi, Institute for Protein Research, Osaka University in 2011. 129 derived ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6.
Strain descriptionLamc1 gene Glu1605Gln variant knock-in mice (cre-inducible).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
Laminin gamma1 C-terminal Glu to Gln mutation induces early postimplantation lethality.
Kiyozumi D, Taniguchi Y, Nakano I, Toga J, Yagi E, Hasuwa H, Ikawa M, Sekiguchi K
Life Science Alliance 1:e201800064, doi: 10.26508/lsa.201800064 30456378

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site1Frt
Lamc1laminin, gamma 11Lamc1targeted mutation 2.1, Kiyotoshi Sekiguchi
loxPphage P1 loxP1loxP
loxPphage P1 loxP1loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Lamc1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Life Science Alliance 1:e201800064, doi: 10.26508/lsa.201800064RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorKiyotoshi Sekiguchi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data