Strain Data Sheet

RBRC06500

Strain Information

Image
BRC No.RBRC06500
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSJL.Cg-Aqp4<tm1.1(GFP)Mysi>/MysiRbrc
Former Common nameSJL-Aqp4, KI/GFPdNeo#5, aquaporin 4 (GFP)-9 (Acc.No. CDB0758K-1)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Yoichiro Abe and Masato Yasui, Scholl of Medicine, Keio University. TT2 ES cells were used to generate the mutant mice. PGK-neo cassette was removed by crossing with B6-CAG-FLPe#37 mice. The mutant mice were backcrossed to SJL/J mice.
Strain descriptionAqp4 gene GFP knock-in mice. GFP was inserted into exon 1. Homozygous mutant mice are viable and fertile. SJL/J background.
Colony maintenanceHomozygote x Homozygote [or Crossing to SJL/J(Crlj)]
References
Immunological function of aquaporin-4 in stab-wounded mouse brain in concert with a pro-inflammatory cytokine inducer, osteopontin.
Ikeshima-Kataoka H, Abe Y, Abe T, Yasui M
Mol Cell Neurosci, 56, 65-75 (2013). 23428384

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aqp4aquaporin 418Aqp4targeted mutation 1.1, Masato Yasui
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)18EGFP
Frtyeast FRT (flippase recombination target) site18Frt
loxPphage P1 loxP18loxP
loxPphage P1 loxP18loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal microglial cell physiology(MP:0003691)

  • cerebral edema(MP:0011854)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, jellyfish GFP cDNA, yeast FRT (flipase recombination target) site, poly A, mouse Aqp4 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Masato Yasui (myasui@a3.keio.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0758K-1) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (未発表) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data