Strain Data Sheet

RBRC06494

update : 2024-09-20

Strain Information
Image
BRC No.	RBRC06494
Type	Targeted Mutation
Species	Mus musculus
Strain name	C57BL/6-Grm6<tm1Nak>
Former Common name
H-2 Haplotype
ES Cell line	CCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain development	Developed by Shigetada Nakanishi, Kyoto University Faculty of Medicine. CCE/EK.CCE ES cells derived from 129S/SvEv-Gpi1<c> were used to generate the mutant mice. C57BL/6 semicongenic.
Strain description	Grm6 gene knock-out mice. mGluR6 (glutamate receptor, metabotropic 6) gene is suggested to be responsible for ON responses in both the rod and cone systems. Homozygous mutant mice are viable and fertile. This strain is useful for investigating the promoter function of the cell-specific and developmentally regulated expression of mGluR6.
Colony maintenance	Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
References	Immunohistological studies of metabotropic glutamate receptor subtype 6-deficient mice show no abnormality of retinal cell organization and ganglion cell maturation. Tagawa Y, Sawai H, Ueda Y, Tauchi M, Nakanishi S J. Neurosci., 19, 2568-2579 (1999). 10087070 Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene. Masu M, Iwakabe H, Tagawa Y, Miyoshi T, Yamashita M, Fukuda Y, Sasaki H, Hiroi K, Nakamura Y, Shigemoto R, et a l Cell, 80, 757-765 (1995). 7889569 The mGluR6 5' upstream transgene sequence directs a cell-specific and developmentally regulated expression in retinal rod and ON-type cone bipolar cells. Ueda Y, Iwakabe H, Masu M, Suzuki M, Nakanishi S J. Neurosci., 17, 3014-3023 (1997). 9096137

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Grm6	glutamate receptor, metabotropic 6	11	Grm6	targeted mutation 1, Shigetada Nakanishi			congenital stationary night blindness 1B(DisGeNET, MedGEN)
neo	neomycin resistance gene (E. coli)	11				herpes simplex virus thymidine kinase promoter (HSV tk promoter)	congenital stationary night blindness 1B(DisGeNET, MedGEN)

Phenotype
Annotation by Mammalian phenotyhpe ontology	abnormal eye electrophysiology(MP:0005551) abnormal synaptic transmission(MP:0003635) behavior/neurological phenotype(MP:0005386)
Detailed phenotype data

Ordering Information
Donor DNA	E. coli neo, herpes simplex virus thymidine kinase promoter (HSV tk promoter), mouse grm6 genomic DNA
Research application
Specific Term and Conditions	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 19, 2568-2579 (1999). J. Neurosci., 17, 3014-3023 (1997). Cell, 80, 757-765 (1995).
Depositor	Shigetada Nakanishi (Kyoto University)
Strain Status	Frozen sperm
Strain Availability	Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month)
Additional Info.	Necessary documents for ordering: Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Genotyping protocol -PCR-

BRC mice in Publications

No Data