Strain Data Sheet

RBRC06468

Strain Information

Image
BRC No.RBRC06468
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Ppp1r17<tm1Nkz>
Former Common nameG-substrate KO, G-substrate KO
H-2 Haplotype
ES Cell lineMS12 [C57BL/6]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shogo Endo, Tokyo Metropolitan Institute of Gerontology. MS12 ES cells derived from C57BL/6 were used to generate the mutant mice. C57BL/6J background.
Strain descriptionG-substrate (Ppp1r17) gene knockout mice. Exon 2 was replaced with a tau-AFAP-PGK-neo cassette. The PGK-neo was subsequently removed via CAG-cre mediated recombination. Homozygous mutant mice exhibit loss of long-term OKR adaptation and temporary loss of cerebellar LTD in early developmental stage. No obvious ataxia or abnormal movement.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Retinal G-substrate, potential downstream component of NO/cGMP/PKG pathway, is located in subtype of retinal ganglion cells and amacrine cells with protein phosphatases.
Nakazawa T, Endo S, Shimura M, Kondo M, Ueno S, Tamai M
Mol. Brain Res., 35(1-2) 58-68 (2005). 15857669

Dual involvement of G-substrate in motor learning revealed by gene deletion.
Endo S, Shutoh F, Dinh T L, Okamoto T, Ikeda T, Suzuki M, Kawahara S, Yanagihara D, Sato Y, Yamada K, Sakamoto T, Kirino Y, Hartell N A, Yamaguchi K, Itohara S, Nairn A C, Greengard P, Nagao S, Ito M
Proc. Natl. Acad. Sci. USA, 106, 3525-3530 (2009). 19218432

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
AFAPGreen Fluorescent Protein (Aequorea victoria)6AFAP
  • hypercholesterolemia, familial, 1(MedGEN)
    • Ppp1r17protein phosphatase 1, regulatory subunit 176Ppp1r17targeted mutation 1, Toru Nakazawa
  • hypercholesterolemia, familial, 1(MedGEN)
    • loxPphage P1 loxP6loxP
  • hypercholesterolemia, familial, 1(MedGEN)
    • taubovine tau protein cDNA6tau
  • hypercholesterolemia, familial, 1(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal long-term depression(MP:0001898)

  • abnormal optokinetic reflex(MP:0009619)

  • absent long-term depression(MP:0001899)

  • vision/eye phenotype(MP:0005391)
    • Detailed phenotype data

    Ordering Information

    Donor DNAbovine tau cDNA, jellyfish GFP derivative (AFAP) cDNA, phage P1 loxP site, mouse Gsbs (Ppp1r17) genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A, 106, 3525-3530 (2009).Required the closing of MTA with Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology.
    DepositorShogo Endo (Tokyo Metropolitan Institute of Gerontology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data