Strain Data Sheet



BRC No.RBRC06467
SpeciesMus musculus
Strain nameC57BL/6-Tg(Prnp-Sirt1,Cryaa-EGFP)1Ksh
Former Common namePrP-Sirt1 Tg, B6-Prnp-Sirt1/Cryaa-GFP Tg
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Makoto Kinoshita, Nagoya University Graduate School of Science (2008-9). Prnp-Sirt1 and alpha A-crystallin EGFP transcription units were co-injected into the C57BL/6J mouse oocytes.
Strain descriptionPrP-Sirt1 transgenic mice having a mouse Sirt1 gene under the control of mouse prion gene promoter (MoPrP). The MoPrP-Sirt1 transcription unit was co-injected with a mouse alpha A-crystallin promoter-driven EGFP cDNA.
Colony maintenanceCarrier x Nocarrier [C57BL/6JJmsSlc]
ReferencesStroke 45, 3403-3411 (2014). 25213338
Mol. Brain, 7(1):62 (2014). 25167838
F1000Res. 4:130 (2015). 26167274

Health Report

Examination Date / Room / Racknull


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPmouse Cryaa promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sirt1sirtuin 1UNSirt1mouse Prnp promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signalUN


Donor DNAMouse Prnp gene promoter, Mouse Sirt1 gene cDNA, Mouse aA-crystallin gene promoter, Aequorea victoria (humanized) enhanced green fluorescent protein (GFP) gene cDNA, SV40 PolyA signal
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol Brain. 2014 Aug 29;7(1):62. doi: 10.1186/s13041-014-0062-1.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For profit use of the BIOLOGICAL RESSOURCE, the RECIPIENT must contact with the Intellectual Property Office, Nagoya University to obtain a prior written consent.
DepositorMakoto Kinoshita (Nagoya University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Feb 2015
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data