Strain Data Sheet

RBRC06467

Strain Information

Image
BRC No.RBRC06467
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Prnp-Sirt1,Cryaa-EGFP)1Ksh
Former Common namePrP-Sirt1 Tg, B6-Prnp-Sirt1/Cryaa-GFP Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Makoto Kinoshita, Nagoya University Graduate School of Science (2008-9). Prnp-Sirt1 and alpha A-crystallin EGFP transcription units were co-injected into the C57BL/6J mouse oocytes.
Strain descriptionPrP-Sirt1 transgenic mice having a mouse Sirt1 gene under the control of mouse prion gene promoter (MoPrP). The MoPrP-Sirt1 transcription unit was co-injected with a mouse alpha A-crystallin promoter-driven EGFP cDNA.
Colony maintenanceCarrier x Nocarrier [C57BL/6JJmsSlc]
References
Transgenic supplementation of SIRT1 fails to alleviate acute loss of nigrostriatal dopamine neurons and gliosis in a mouse model of MPTP-induced parkinsonism.
Kitao Y, Ageta-Ishihara N, Takahashi R, Kinoshita M, Hori O
F1000Res. 4:130 (2015). 26167274

SIRT1 overexpression ameliorates a mouse model of SOD1-linked amyotrophic lateral sclerosis via HSF1/HSP70i chaperone system.
Watanabe S, Ageta-Ishihara N, Nagatsu S, Takao K, Komine O, Endo F, Miyakawa T, Misawa H, Takahashi R, Kinoshita M, Yamanaka K
Mol. Brain, 7(1):62 (2014). 25167838

Silent information regulator 2 homolog 1 counters cerebral hypoperfusion injury by deacetylating endothelial nitric oxide synthase.
Hattori Y, Okamoto Y, Maki T, Yamamoto Y, Oishi N, Yamahara K, Nagatsuka K, Takahashi R, Kalaria R N, Fukuyama H, Kinoshita M, Ihara M
Stroke 45, 3403-3411 (2014). 25213338

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signalUN
  • cataract 9 multiple types(DisGeNET, MedGEN)

  • fatal familial insomnia(DisGeNET, MedGEN)

  • Gerstmann-Straussler-Scheinker syndrome(DisGeNET, MedGEN)
    • more 4 Diseases
  • Huntington disease-like 1(MedGEN)

  • inherited Creutzfeldt-Jakob disease(MedGEN)

  • kuru, susceptibility to(MedGEN)

  • spongiform encephalopathy with neuropsychiatric features(MedGEN)
    • EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPmouse Cryaa promoter
  • cataract 9 multiple types(DisGeNET, MedGEN)

  • fatal familial insomnia(DisGeNET, MedGEN)

  • Gerstmann-Straussler-Scheinker syndrome(DisGeNET, MedGEN)
    • more 4 Diseases
  • Huntington disease-like 1(MedGEN)

  • inherited Creutzfeldt-Jakob disease(MedGEN)

  • kuru, susceptibility to(MedGEN)

  • spongiform encephalopathy with neuropsychiatric features(MedGEN)
    • Sirt1sirtuin 1UNSirt1mouse Prnp promoter
  • cataract 9 multiple types(DisGeNET, MedGEN)

  • fatal familial insomnia(DisGeNET, MedGEN)

  • Gerstmann-Straussler-Scheinker syndrome(DisGeNET, MedGEN)
    • more 4 Diseases
  • Huntington disease-like 1(MedGEN)

  • inherited Creutzfeldt-Jakob disease(MedGEN)

  • kuru, susceptibility to(MedGEN)

  • spongiform encephalopathy with neuropsychiatric features(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased body weight(MP:0001262)

  • enhanced active avoidance behavior(MP:0012294)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Prnp gene promoter, Mouse Sirt1 gene cDNA, Mouse aA-crystallin gene promoter, Aequorea victoria (humanized) enhanced green fluorescent protein (GFP) gene cDNA, SV40 PolyA signal
    Research applicationFluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Brain, 7(1):62 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For profit use of the BIOLOGICAL RESSOURCE, the RECIPIENT must contact with the Intellectual Property Office, Nagoya University to obtain a prior written consent.
    DepositorMakoto Kinoshita (Nagoya University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Feb 2015

    BRC mice in Publications

    No Data