Strain Data Sheet
RBRC06462
Information1 | |
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| Image | |
| BRC No. | RBRC06462 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Prkcd<tm1Shb> |
| Former Common name | PKCdelta floxed, B6-Prkcd floxed |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Yuko Niino and Seiji Shiota, School of Medicine, Showa University and Kenji Skimura, Brain Research Institute, Niigata University. RENKA ES cells were used to generate the mutant mice. |
| Strain description | Prkcd gene floxed mice. A loxP site and PGK-neo cassette were inserted upstream of exon 7, and a loxP site was inserted downstream of exon 7. Under investigation. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj] |
| References | PLoS One, 16(7):e0253912 (2021). 34197550 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site14Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site14Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse PGK poly(A), Phage P1 LoxP sites, yeast FRT (flippase recombination target) sites, mouse PKCdelta genomic DNA |
| Research application | Cre/loxP system FLP/frt system Neurobiology Research |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 2021 Jul 1;16(7):e0253912.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR/DEVELOPER (Drs. Seiji Shiota and Yuko Niino) is requested (will be announced). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR/DEVELOPER and its colleagues (Drs. Tomoya Nakamachi and Jun Watanabe) is requested. Before the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR/DEVELOPER, the RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER, and then include them as co-authors in the publication. |
| Depositor | Seiji Shiota (Showa University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |