Strain Data Sheet

RBRC06462

Strain Information

Image
BRC No.RBRC06462
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Prkcd<tm1Shb>
Former Common namePKCdelta floxed, B6-Prkcd floxed
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Yuko Niino and Seiji Shiota, School of Medicine, Showa University and Kenji Skimura, Brain Research Institute, Niigata University. RENKA ES cells were used to generate the mutant mice.
Strain descriptionPrkcd gene floxed mice. A loxP site and PGK-neo cassette were inserted upstream of exon 7, and a loxP site was inserted downstream of exon 7. Under investigation.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
References
PKCdelta deficiency inhibits fetal development and is associated with heart elastic fiber hyperplasia and lung inflammation in adult PKCdelta knockout mice.
Niino Y S, Kawashima I, Iguchi Y, Kanda H, Ogura K, Mita-Yoshida K, Ono T, Yamazaki M, Sakimura K, Yogosawa S, Yoshida K, Shioda S, Gotoh T
PLoS One, 16(7):e0253912 (2021). 34197550

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site14Frt
  • autoimmune lymphoproliferative syndrome, type III caused by mutation in PRKCD(MedGEN)
    • Frtyeast FRT (flippase recombination target) site14Frt
  • autoimmune lymphoproliferative syndrome, type III caused by mutation in PRKCD(MedGEN)
    • Prkcdprotein kinase C, delta14Prkcdtargeted mutation 1, Seiji Shiota
  • autoimmune lymphoproliferative syndrome, type III caused by mutation in PRKCD(MedGEN)
    • loxPphage P1 loxP14loxP
  • autoimmune lymphoproliferative syndrome, type III caused by mutation in PRKCD(MedGEN)
    • loxPphage P1 loxP14loxP
  • autoimmune lymphoproliferative syndrome, type III caused by mutation in PRKCD(MedGEN)
    • neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)
  • autoimmune lymphoproliferative syndrome, type III caused by mutation in PRKCD(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse PGK poly(A), Phage P1 LoxP sites, yeast FRT (flippase recombination target) sites, mouse PKCdelta genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 16(7):e0253912 (2021).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR/DEVELOPER (Drs. Seiji Shiota and Yuko Niino) is requested (will be announced). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR/DEVELOPER and its colleagues (Drs. Tomoya Nakamachi and Jun Watanabe) is requested. Before the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR/DEVELOPER, the RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER, and then include them as co-authors in the publication.
    DepositorSeiji Shiota (Showa University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data