Strain Data Sheet

RBRC06462

Information1

Image
BRC No.RBRC06462
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Prkcd<tm1Shb>
Former Common namePKCdelta floxed, B6-Prkcd floxed
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Yuko Niino and Seiji Shiota, School of Medicine, Showa University and Kenji Skimura, Brain Research Institute, Niigata University. RENKA ES cells were used to generate the mutant mice.
Strain descriptionPrkcd gene floxed mice. A loxP site and PGK-neo cassette were inserted upstream of exon 7, and a loxP site was inserted downstream of exon 7. Under investigation.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
ReferencesPLoS One, 16(7):e0253912 (2021). 34197550

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prkcdprotein kinase C, delta14Prkcd<tm1Shb>targeted mutation 1, Seiji Shiota

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse PGK poly(A), Phage P1 LoxP sites, yeast FRT (flippase recombination target) sites, mouse PKCdelta genomic DNA
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 2021 Jul 1;16(7):e0253912.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR/DEVELOPER (Drs. Seiji Shiota and Yuko Niino) is requested (will be announced). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR/DEVELOPER and its colleagues (Drs. Tomoya Nakamachi and Jun Watanabe) is requested. Before the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR/DEVELOPER, the RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER, and then include them as co-authors in the publication.
DepositorSeiji Shiota (Showa University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data