RBRC06448

Information1

Image
BRC No.RBRC06448
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Clmp<tm1Jwada>
Former Common nameB6/ICR-ACAM KO Clmp KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Jun Wada, Okayama University Graduate School of Medicine, Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo (Research Institute for Biomedical Sciences, Tokyo University of Science) in 2008. 129P2/OlaHsd derived ES cells were used to generate the mutant mice.
Strain descriptionACAM (CLMP) gene knockout mice. Exons 3 and 4 of the ACAM gene was replaced with a floxed-neo cassette. Homozygous mutant mice of C57BL/6 background show a severe growth defect and die by about 6 weeks of age. Homozygous mutant mice of ICR background show and increased birth rate, but die by 14 weeks of age. C57BL/6 background (RBRC06446), ICR background (RBRC06447), B6/ICR mixed (RBRC06448).
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6JJcl and Jcl:ICR]
ReferencesJ Lipid Res ,41, 1615-1622 (2000). 11013303
Biochemical J, 387, 343-353 (2005). 15563274

Health Report

Examination Date / Room / Rack2015/10/05Room:3-5Rack:B
2015/08/03Room:3-5Rack:B
2015/06/01Room:3-5Rack:B
2015/04/06Room:3-7Rack:B
2015/02/02Room:3-7Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
ClmpCXADR-like membrane protein9Clmp<tm1Jwada>targeted mutation 1, Jun Wada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)9GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, bovine Growth hormone polyadenylation signal, mouse Clmp genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorJun Wada (Okayama University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data