Strain Data Sheet

RBRC06446

Strain Information

Image
BRC No.RBRC06446
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Clmp<tm1Jwada>
Former Common nameB6-ACAM KO B6-Clmp KO
H-2 Haplotype
ES Cell lineNot specified (ES cell)
Background strain
Appearance
Strain developmentDeveloped by Jun Wada, Okayama University Graduate School of Medicine, Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo (Research Institute for Biomedical Sciences, Tokyo University of Science) in 2008. 129P2/OlaHsd derived ES cells were used to generate the mutant mice.
Strain descriptionACAM (CLMP) gene knockout mice. Exons 3 and 4 of the ACAM gene was replaced with a floxed-neo cassette. Homozygous mutant mice of C57BL/6 background show a severe growth defect and die by about 6 weeks of age. Homozygous mutant mice of ICR background show and increased birth rate, but die by 14 weeks of age. C57BL/6 background (RBRC06446), ICR background (RBRC06447), B6/ICR mixed (RBRC06448).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Identification of genes specifically expressed in the accumulated visceral adipose tissue of OLETF rats.
Hida K, Wada J, Zhang H, Hiragushi K, Tsuchiyama Y, Shikata K, Makino H
J Lipid Res, 41, 1615-1622 (2000). 11013303

Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity.
Eguchi J, Wada J, Hida K, Zhang H, Matsuoka T, Baba M, Hashimoto I, Shikata K, Ogawa N, Makino H
Biochemical J, 387, 343-353 (2005). 15563274

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ClmpCXADR-like membrane protein9Clmptargeted mutation 1, Jun Wada
  • congenital short bowel syndrome, autosomal recessive(MedGEN)
    • GHGrowth hormone polyA (Bovine)9GH
  • congenital short bowel syndrome, autosomal recessive(MedGEN)
    • loxPphage P1 loxP9loxP
  • congenital short bowel syndrome, autosomal recessive(MedGEN)
    • loxPphage P1 loxP9loxP
  • congenital short bowel syndrome, autosomal recessive(MedGEN)
    • neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)
  • congenital short bowel syndrome, autosomal recessive(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal intestinal smooth muscle morphology(MP:0008802)

  • abnormal intestine placement(MP:0014023)

  • abnormal small intestine morphology(MP:0000496)

  • decreased survivor rate(MP:0008770)

  • increased intestine length(MP:0013954)
    • more 3 phenotypes
  • kidney cyst(MP:0003675)

  • lung cyst(MP:0004880)

  • postnatal lethality, incomplete penetrance(MP:0011086)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, bovine Growth hormone polyadenylation signal, mouse Clmp genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorJun Wada (Okayama University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data