Strain Data Sheet

RBRC06445

Strain Information

Image
BRC No.RBRC06445
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Fabp4-Clmp)22Jwada
Former Common nameACAM Tg#22 B6-aP2-ACAM Tg#22
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Jun Wada, Okayama University Graduate School of Medicine in 2005. C57BL/6J background.
Strain descriptionACAM (CLMP) transgenic mice having a mouse ACAM cDNA under control of the aP2 promoter. Line 4 (RBRC06443), Line 11 (RBRC06444), Line 22 (main line) (RBRC06445).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Identification of genes specifically expressed in the accumulated visceral adipose tissue of OLETF rats.
Hida K, Wada J, Zhang H, Hiragushi K, Tsuchiyama Y, Shikata K, Makino H
J Lipid Res, 41, 1615-1622 (2000). 11013303

Antiobesity Action of ACAM by Modulating the Dynamics of Cell Adhesion and Actin Polymerization in Adipocytes.
Murakami K, Eguchi J, Hida K, Nakatsuka A, Katayama A, Sakurai M, Choshi H, Furutani M, Ogawa D, Takei K, Otsuka F, Wada J
Diabetes, 65(5):1255-67 (2016). 26956488

Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity.
Eguchi J, Wada J, Hida K, Zhang H, Matsuoka T, Baba M, Hashimoto I, Shikata K, Ogawa N, Makino H
Biochemical J, 387, 343-353 (2005). 15563274

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ClmpCXADR-like membrane proteinUNClmpmouse fatty acid binding protein 4 (Fabp4) adipocyte promoter
  • congenital short bowel syndrome, autosomal recessive(MedGEN)
    • GHGrowth hormone polyA (human)UNGH
  • congenital short bowel syndrome, autosomal recessive(MedGEN)
    • HBB2rabbit beta-globin intronUNHBB2
  • congenital short bowel syndrome, autosomal recessive(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased body weight(MP:0001262)

  • decreased brown adipose tissue mass(MP:0014169)

  • decreased brown fat cell lipid droplet size(MP:0009125)

  • decreased brown fat lipid droplet number(MP:0009137)

  • decreased circulating LDL cholesterol level(MP:0000183)
    • more 6 phenotypes
  • decreased fat cell size(MP:0009269)

  • decreased total fat pad weight(MP:0008907)

  • improved glucose tolerance(MP:0005292)

  • increased insulin sensitivity(MP:0002891)

  • increased oxygen consumption(MP:0005289)

  • insulin resistance(MP:0005331)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse fatty acid binding protein 4 (Fabp4) adipocyte promoter, rabbit beta-globin intron, mouse (CLMP) ACAM gene cDNA, human GH polyA signal
    Research applicationDiabetes and Obesity Research
    covid19_progression
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorJun Wada (Okayama University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data