Strain Data Sheet


Strain Information

BRC No.RBRC06436
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Pemt<tm1>
Former Common nameB6-Pemt-/-, B6-Pemt KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Strain developmentDeveloped by Jun Wada, Okayama University Graduate School of Medicine, Eijiro Watanabe, Dainippon Sumitomo Phama Co. Ltd. C57BL/6 derived ES cells were used to generate the mutant mice.
Strain descriptionPemt gene knockout mice. Exon 2 of the Pemt gene was replaced with a neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesBiochimica et Biophysica Acta. 1831:626-632 (2013). 22877991
PLoS One 9(3):e92647, (2014). 24667182

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pemtphosphatidylethanolamine N-methyltransferase11Pemt<tm1Jwada>targeted mutation 1, Jun Wada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11

Ordering Information

Donor DNAMouse PGK promoter (マウス由来ゲノムDNA), E. coli ネオマイシン リン酸塩基転移酵素(大腸菌由来cDNA), Mouse PGK poly A (マウス由来ゲノムDNA), Simian virus SV40 poly A (Simian virus 40由来ゲノムDNA),バクテリオファージ loxP配列(バクテイオファージP1由来ゲノムDNA)
Research applicationCre/loxP system
Diabetes and Obesity Research
Metabolism Research
Mouse Models for Human Disease
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorJun Wada (Okayama University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data