Strain Data Sheet

RBRC06436

Strain Information

Image
BRC No.RBRC06436
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Pemt<tm1Jwada>
Former Common nameB6-Pemt-/-, B6-Pemt KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Jun Wada, Okayama University Graduate School of Medicine, Eijiro Watanabe, Dainippon Sumitomo Phama Co. Ltd. C57BL/6 derived ES cells were used to generate the mutant mice.
Strain descriptionPemt gene knockout mice. Exon 2 of the Pemt gene was replaced with a neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Physiological roles of phosphatidylethanolamine N-methyltransferase.
Vance D E
Biochimica et Biophysica Acta. 1831:626-632 (2013). 22877991

Pemt deficiency ameliorates endoplasmic reticulum stress in diabetic nephropathy.
Watanabe M, Nakatsuka A, Murakami K, Inoue K, Terami T, Higuchi C, Katayama A, Teshigawara S, Eguchi J, Ogawa D, Watanabe E, Wada J, Makino H
PLoS One 9(3):e92647, (2014). 24667182

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pemtphosphatidylethanolamine N-methyltransferase11Pemttargeted mutation 1, Jun Wada
loxPphage P1 loxP11loxP
loxPphage P1 loxP11loxP
neoneomycin resistance gene (E. coli)11mouse Phosphoglycerate kinase (PGK) promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal endoplasmic reticulum stress(MP:0020353)

  • abnormal lipid metabolism(MP:0013245)

  • abnormal small intestine morphology(MP:0000496)

  • albuminuria(MP:0002871)

  • decreased cellular sensitivity to oxidative stress(MP:0008413)
    • more 8 phenotypes
  • decreased circulating homocysteine level(MP:0012334)

  • decreased macrophage cell number(MP:0003884)

  • decreased renal tubule apoptosis(MP:0011372)

  • decreased susceptibility to diet-induced obesity(MP:0005659)

  • decreased transforming growth factor beta level(MP:0008838)

  • mesangiolysis(MP:0011430)

  • renal glomerulus hypertrophy(MP:0011294)

  • small intestinal villus atrophy(MP:0014078)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse PGK promoter, E. coli neomycin resistance gene, mouse PGK polyA, Simian virus SV40 polyA, mouse Pemt genomic DNA
    Research applicationCre/loxP system
    Diabetes and Obesity Research
    Metabolism Research
    Mouse Models for Human Disease
    covid19_progression
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorJun Wada (Okayama University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data