RBRC06427

Information1

Image
BRC No.RBRC06427
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm1(CAG-SCAT1)Miur>
Former Common nameRosa26-CAG-STOP-SCAT1, B6-ROSA26/SCAT1floxed KI
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Ting Liu, Yoshifumi Yamaguchi and Masayuki Miura, Graduate School of Pharmaceutical Sciences, University of Tokyo, and Katsuaki Hoshino and Tsuneyasu Kaisho, RIKEN Research Center for Allergy and Immunology. C57BL/6J and C57BL/6N mixed.
Strain descriptionCAG-STOP-CAST1 knock-in mice. A CAG-loxP-neo-loxP-SCAT1-pA cassette was inserted into the Gt(ROSA)26Sor locus. Homozygous mice are viable (fertility is unknown).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesCell Rep., 8(4):974-982 (2014). 25127135

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-SCAT1)Miur>targeted mutation 1, Masayuki Miura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
ECFPEnhanced Cyan Fluorescent Protein (Aequorea victoria), F64L/S65T/K212N/H231L6ECFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)6Venus

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal6

Information2

Donor DNACAG promoter (CMV-IE enhancer,chicken beta-actin promoter, rabbit beta-globin genomic DNA), SCAT1 (Jellyfish ECFP cDNA, caspase-1 cleavage site, Jellyfish Venus cDNA), herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neomycin resistance gene, phage P1 loxP sites, Bovine polyA, SV40 polyA, yeast FRT (flipase recombination target) site, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Single-cell imaging of caspase-1 dynamics reveals an all-or-none inflammasome signaling response” Cell Reports, 8(4):974-982 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorMasayuki Miura (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)

BRC mice in Publications

No Data