Strain Data Sheet

RBRC06410

Strain Information

Image
BRC No.RBRC06410
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nfkbiz<tm1.1Muta>
Former Common nameIκB-ζ flox/flox, B6-Nfkbiz floxed
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Tatsushi Muta, Graduate School of Life Sciences, Tohoku University. Bruce 4 ES cells were used to generate the mutant mice. A frt-neo cassette was removed by crossing with CAG-FLPe (B6) mice.
Strain descriptionNfkbiz gene floxed mice. Exons 5 to 7 were flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
References
Enhanced apoptosis by disruption of the STAT3-IκB-ζ signaling pathway in epithelial cells induces Sjögren's syndrome-like autoimmune disease.
Atsushi Okuma, Katsuaki Hoshino, Tomoyuki Ohba, Sawako Fukushi, Setsuya Aiba, Shizuo Akira, Masao Ono, Tsuneyasu Kaisho, Tatsushi Muta
Immunity, 38, 450-460 (2013). 23453632

Health Report

Examination Date / Room / Rack2025/11/10Room:3-BRack:LSentinel mouse program
2025/09/01Room:3-BRack:LSentinel mouse program
2025/06/16Room:3-BRack:LSentinel mouse program
2025/04/07Room:3-BRack:LSentinel mouse program
2025/01/07Room:3-BRack:LSentinel mouse program
2024/10/07Room:3-BRack:LSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site16Frt
Nfkbiz
MGI:1931595		nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta16Nfkbiz<tm1.1Muta>
MGI:5501251		targeted mutation 1.1, Tatsushi Muta
loxP phage P1 loxP16loxP
loxP phage P1 loxP16loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP sites, yeast FRT (flippase recombination target) site, mouse Nfkbiz genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 38, 450-460 (2013). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorTakashi Maruyama (Gifu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Apr 2017

    BRC mice in Publications

    Iguchi E, Takai A, Oe N, Fujii Y, Omatsu M, Takeda H, Shimizu T, Maruno T, Nakanishi Y, Yoshinaga M, Maruyama T, Marusawa H, Obama K, Takeuchi O, Seno H.
    Epithelial Regnase-1 inhibits colorectal tumor growth by regulating IL-17 signaling via degradation of NFKBIZ mRNA.
    Proc Natl Acad Sci U S A 122(23) e2500820122(2025) 40460118
    Taylor TC, Coleman BM, Arunkumar SP, Dey I, Dillon JT, Ponde NO, Poholek AC, Schwartz DM, McGeachy MJ, Conti HR, Gaffen SL.
    IκBζ is an essential mediator of immunity to oropharyngeal candidiasis.
    Cell Host Microbe (2023) 37725983
    Terui H, Yamasaki K, Wada-Irimada M, Onodera-Amagai M, Hatchome N, Mizuashi M, Yamashita R, Kawabe T, Ishii N, Abe T, Asano Y, Aiba S.
    Staphylococcus aureus skin colonization promotes SLE-like autoimmune inflammation via neutrophil activation and the IL-23/IL-17 axis.
    Sci Immunol 7(76) eabm9811(2022) 36306369
    Ishikawa H, Hayakawa M, Baatartsogt N, Kakizawa N, Ohto-Ozaki H, Maruyama T, Miura K, Suzuki K, Rikiyama T, Ohmori T.
    IκBζ regulates the development of nonalcoholic fatty liver disease through the attenuation of hepatic steatosis in mice.
    Sci Rep 12(1) 11634(2022) 35804007
    Murakami T, Takahata Y, Hata K, Ebina K, Hirose K, Ruengsinpinya L, Nakaminami Y, Etani Y, Kobayashi S, Maruyama T, Nakano H, Kaneko T, Toyosawa S, Asahara H, Nishimura R.
    Semaphorin 4D induces articular cartilage destruction and inflammation in joints by transcriptionally reprogramming chondrocytes.
    Sci Signal 15(758) eabl5304(2022) 36318619
    Arra M, Swarnkar G, Ke K, Otero JE, Ying J, Duan X, Maruyama T, Rai MF, O'Keefe RJ, Mbalaviele G, Shen J, Abu-Amer Y.
    LDHA-mediated ROS generation in chondrocytes is a potential therapeutic target for osteoarthritis.
    Nat Commun 11(1) 3427(2020) 32647171
    Kakiuchi N, Yoshida K, Uchino M, Kihara T, Akaki K, Inoue Y, Kawada K, Nagayama S, Yokoyama A, Yamamoto S, Matsuura M, Horimatsu T, Hirano T, Goto N, Takeuchi Y, Ochi Y, Shiozawa Y, Kogure Y, Watatani Y, Fujii Y, Kim SK, Kon A, Kataoka K, Yoshizato T, Nakagawa MM, Yoda A, Nanya Y, Makishima H, Shiraishi Y, Chiba K, Tanaka H, Sanada M, Sugihara E, Sato TA, Maruyama T, Miyoshi H, Taketo MM, Oishi J, Inagaki R, Ueda Y, Okamoto S, Okajima H, Sakai Y, Sakurai T, Haga H, Hirota S, Ikeuchi H, Nakase H, Marusawa H, Chiba T, Takeuchi O, Miyano S, Seno H, Ogawa S.
    Frequent mutations that converge on the NFKBIZ pathway in ulcerative colitis.
    Nature 577(7789) 260-265(2020) 31853061