RBRC06404

Information1

Image
BRC No.RBRC06404
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Cdkn1c<tm1.1(Cdkn1b*)Kei>
Former Common namep57/p27KI, B6-Cdkn1c/Cdkn1b KI
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J over 5 times.
Strain descriptionp57 gene p27 knockin mice. Intron 2 of p57 was replaced with a p27 cDNA (p57<p27KI> type). Paternal imprinting gene.
Colony maintenanceHeterozygote (male) x Wild-type (female) [C57BL/6JJcl]
ReferencesProc. Natl. Acad. Sci. USA, 106, 5192-5197 (2009). 19276117

Health Report

Examination Date / Room / Rack2016/01/06Room:4-ARack:E
2016/01/04Room:4-ARack:E
2015/11/30Room:4-ARack:E
2015/10/07Room:4-ARack:E
2015/10/05Room:4-ARack:E
2015/09/28Room:4-ARack:E
2015/08/19Room:4-ARack:E
2015/08/17Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cdkn1ccyclin-dependent kinase inhibitor 1C (P57)7Cdkn1c<tm1.1(Cdkn1b*)Kei>targeted mutation 1.1, Keiichi I Nakayama

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Information2

Donor DNAphage P1 LoxP site, mouse Cdkn1b cDNA, mouse Cdkn1c genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data