RBRC06397

Information1

Image
BRC No.RBRC06397
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Fkbp8<tm1Kei>
Former Common nameFkbp38 KO, B6-Fkbp38 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J over 7 times.
Strain descriptionFkbp38 gene knockout mice. Exons 3 to 7 were raplaced with a PGK-neo (loxP-neo) cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesGenes to Cells, 13, 635-651 (2008). 18459960

Health Report

Examination Date / Room / Rack2018/09/25Room:4-ARack:H
2018/08/20Room:4-ARack:H
2018/07/30Room:4-ARack:H
2018/06/11Room:4-ARack:H
2018/06/11Room:4-ARack:H
2018/05/21Room:4-ARack:H
2018/03/26Room:4-ARack:H
2018/01/22Room:4-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Fkbp8FK506 binding protein 88Fkbp8<tm1Kei>targeted mutation 1, Keiichi I Nakayama

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal8

Information2

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, SV40 polyA signal, mouse Fkbp8 genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data