Strain Data Sheet

RBRC06394

Information1

Image
BRC No.RBRC06394
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Fbxw7<tm1Kin>
Former Common nameFbxw7 KO, B6-Fbxw7 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J over 7 times.
Strain descriptionFbxw7 gene knockout mice. Exons 1 to 5 was replaced with PGK-neo (loxP-neo) cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesJ. Biol. Chem., 279, 9417-9423 (2004). 14672936

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Fbxw7F-box and WD-40 domain protein 73Fbxw7<tm1Kin>targeted mutation 1, Kei-ichi Nakayama

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal3

Information2

Donor DNAE. coli Neomycin resistance gene, mouse phosphoglycerate kinase promoter, SV40 polyA signal, phage P1 loxP
Research applicationHematological Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Mouse of the Month Jan 2015
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data