RBRC06391

Information1

Image
BRC No.RBRC06391
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Fbxo4<tm1Kei>
Former Common nameFbxo4 KO, B6-Fbxo4 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J.
Strain descriptionFbxo4 gene knockout mice. Exons 4 to 5 were raplaced with an IRES-lacZ/neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesMol. Cell Biol., 32, 590-605 (2012). 22124152

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Fbxo4F-box protein 415Fbxo4<tm1Kei>targeted mutation 1, Keiichi I Nakayama

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)15

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)15

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)15mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal15

Information2

Donor DNAE. coli Neomycin resistance gene, mouse phosphoglycerate kinase promoter, SV40 polyA signal, ECMV internal ribosomal entry site (ires), E. coli lacZ, phage P1 loxP
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data