RBRC06389

Information1

Image
BRC No.RBRC06389
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Fbxl3<tm1Kei>
Former Common nameFbxl3 KO, B6-Fbxl3 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J (N7 generations).
Strain descriptionFbxl3 gene knockout mice. Exons 2 and 3 were replaced with an IRES-lacZ/neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesCell, 152, 1106-1118 (2013). 23452856

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Fbxl3F-box and leucine-rich repeat protein 314Fbxl3<tm1Kei>targeted mutation 1, Keiichi I Nakayama

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)14

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)14

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal14

Information2

Donor DNAE. coli Neomycin resistance gene, mouse phosphoglycerate kinase promoter, SV40 polyA signal, ECMV internal ribosomal entry site (ires), E. coli lacZ, phage P1 loxP
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Metabolism Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data